Article abstract
Nature Cell Biology 2, 435 - 440 (2000)
Published online: 9 June 2000 | doi:10.1038/35017072
Modulation of retinoid signalling through NGF-induced nuclear export of NGFI-B
Yasuhiro Katagiri1,4, Kazuyo Takeda2, Zu-Xi Yu2, Victor J. Ferrans2, Keiko Ozato3 & Gordon Guroff1
Abstract
The retinoid–X receptor (RXR) regulates multiple hormonal pathways through heterodimerization with nuclear receptors such as the all-trans retinoic acid receptor (RAR). The orphan nuclear receptor NGFI-B (also called Nur77) can heterodimerize with RXR. Here we show that nerve growth factor (NGF) induces the phosphorylation of Ser 105 of NGFI-B in PC12 phaeochromocytoma cells, resulting in translocation of the NGFI-B–RXR heterodimer complex out of the nucleus using nuclear export signals within NGFI-B. As a consequence of the redistribution of RXR, the transcriptional activity of RXR–RAR is reduced. NGFI-B-mediated nuclear export of receptors may serve as a mechanism for crosstalk between NGF and retinoid pathways.
- Section on Growth Factors, NICHD, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
- Pathology Section, NHLBI, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
- Laboratory of Molecular Growth Regulation, NICHD, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA
- Current address: American Red Cross, Holland Laboratory, Biochemistry Department, 15601 Crabbs Branch Way, Rockville, Maryland 20892, USA
Correspondence to: Yasuhiro Katagiri1,4 e-mail: katagiriy@usa.redcross.org
