These authors contributed equally to this work.
- Marta N. Shahbazi,
- Agnieszka Jedrusik &
- Sanna Vuoristo
Present address: Bioimaging and Optofluidics group, IOGS, CNRS & University of Bordeaux Rue Francois Mitterand, 33400 Talence, France.
- Gaelle Recher
Mammalian Embryo and Stem Cell Group, University of Cambridge, Department of Physiology, Development and Neuroscience, Downing Street, Cambridge CB2 3DY, UK
- Marta N. Shahbazi,
- Agnieszka Jedrusik,
- Sanna Vuoristo,
- Gaelle Recher,
- Anna Hupalowska &
- Magdalena Zernicka-Goetz
Faculty of Life Sciences and Medicine, King’s College London, Women’s Health Academic Centre, Assisted Conception Unit, Guy’s Hospital, Great Maze Pond, London SE1 9RT, UK
- Virginia Bolton,
- Liani G. Devito,
- Dusko Ilic &
- Yakoub Khalaf
Human Embryo and Stem Cell Laboratory, Francis Crick Institute, Mill Hill Laboratory, London NW7 1AA, UK
- Norah M. E. Fogarty &
- Kathy K. Niakan
CARE Fertility Group, John Webster House, 6 Lawrence Drive, Nottingham Business Park, Nottingham NG8 6PZ, UK
- Alison Campbell &
- Simon Fishel
M.N.S., A.J. and S.V. carried out all experiments and data analyses. G.R. analysed microscopy data and generated 3D reconstructions. A.H. prepared illustrations and contributed experimentally. N.M.E.F. and K.K.N. helped with human embryo cultures and contributed experimentally, and L.G.D. helped with human embryo cultures. A.C., S.F., D.I., Y.K. and K.K.N. oversaw and provided human embryos for these studies. M.Z.-G. conceived the project and supervised the study. M.N.S. and M.Z.-G. wrote the manuscript with help from all of the authors.
Competing financial interests
The authors declare no competing financial interests.
Marta N. Shahbazi
Norah M. E. Fogarty
Liani G. Devito
Kathy K. Niakan
Supplementary Figure 1: 3D analysis of embryonic and extra-embryonic lineages in human embryos cultured through implantation stages.Hover over figure to zoom
Supplementary Figure 2: Confocal Z-sections of a day 7–8 human embryo.Hover over figure to zoom
Supplementary Figure 3: Confocal Z-sections of a day 8–9 human embryo.Hover over figure to zoom
Supplementary Figure 4: Confocal Z-sections of a day 9–10 human embryo.Hover over figure to zoom
Supplementary Figure 5: Confocal Z-sections of a day 10–11 human embryo.Hover over figure to zoom
Video 1: Development of a day 5 human blastocyst in the in vitro culture system up to day 9–10.
Video 2: Development of a day 9 human blastocyst in the in vitro culture system up to day 12.
Video 3: 3D reconstruction of embryonic lineages in a day 9–10 human embryo cultured in vitro.
Video 4: 3D reconstruction of embryonic lineages in a day 10–11 human embryo cultured in vitro.
Video 5: 3D reconstruction of the cellular and nuclear shape of representative trophectoderm cells at day 10–11.
Video 6: 3D reconstruction of the pro-amniotic cavity at day 9–10.
Video 7: 3D reconstruction of the cellular shape of representative OCT4-expressing epiblast cells at day 10–11.
Video 8: 3D reconstruction of the prospective yolk sac at day 10–11.
Video 9: 3D reconstruction of the hypoblast derived cells and their position with respect to the prospective yolk sac at day 10–11.
Supplementary Table 1: Summary of human embryos cultured in this study.Hover over figure to zoom
Supplementary Table 2: Average number of epiblast and hypoblast cells in human embryos at different stages of implantation development in vitro.Hover over figure to zoom