How robust are your data? - p667

doi:10.1038/ncb0609-667a

New rules for the presentation of statistics.

Full Text - How robust are your data? | PDF (235 KB) - How robust are your data?

Attribution and accountability - p667

doi:10.1038/ncb0609-667b

Author contribution statements are now mandatory and author responsibilities have been clarified.

Full Text - Attribution and accountability | PDF (235 KB) - Attribution and accountability

Reproducible methods - p667

doi:10.1038/ncb0609-667c

Nature Cell Biology will publish online methods in more detail.

Full Text - Reproducible methods | PDF (235 KB) - Reproducible methods

New fashion models - p668

Christian Braendle & Marie-Anne Félix review Emerging Model Organisms: A Laboratory Manual, volume 1

doi:10.1038/ncb0609-668

Full Text - New fashion models | PDF (219 KB) - New fashion models

STIMulating store-operated Ca²⁺ entry - pp669 - 677

Michael D. Cahalan

doi:10.1038/ncb0609-669

Abstract - | Full Text - STIMulating store-operated Ca2+ entry | PDF (856 KB) - STIMulating store-operated Ca2+ entry | Supplementary information

Wicked views on stem cell news - pp678 - 679

Ralph A. Neumüller & Juergen A. Knoblich

doi:10.1038/ncb0609-678

Stem cells achieve the remarkable task of generating both identical copies of themselves and lineage-restricted daughter cells that ultimately undergo terminal differentiation. The differential regulation of ribosomal protein biosynthesis helps to generate these two outcomes in Drosophila melanogaster.

Full Text - Wicked views on stem cell news | PDF (471 KB) - Wicked views on stem cell news

See also: Article by Fichelson et al.

Inverted rod nuclei see the light - pp680 - 681

Christopher Eskiw & Peter Fraser

doi:10.1038/ncb0609-680

Eighty years of microscopy have established a conventional view of nuclear organization: dark-staining heterochromatin at the nuclear periphery and light-staining euchromatin in the interior. This nuclear architecture is inverted in rod cells of nocturnal mammals, demonstrating a unique functional nuclear genome reorganization specifically adapted for light transmission.

Full Text - Inverted rod nuclei see the light | PDF (303 KB) - Inverted rod nuclei see the light

MicroRNA-mediated regulation of synaptic palmitoylation: shrinking fat spines - pp681 - 682

Damien Carrel & Bonnie L. Firestein

doi:10.1038/ncb0609-681

Local regulation of protein translation is essential for synaptic plasticity. MicroRNA-mediated alteration in expression of an enzyme that regulates the palmitoylation of a specific synaptic protein determines dendritic spine size.

Full Text - MicroRNA-mediated regulation of synaptic palmitoylation: shrinking fat spines | PDF (411 KB) - MicroRNA-mediated regulation of synaptic palmitoylation: shrinking fat spines

See also: Article by Siegel et al.

Research highlights - p684

doi:10.1038/ncb0609-684

Full Text - Research highlights | PDF (281 KB) - Research highlights

Live-imaging of single stem cells within their niche reveals that a U3snoRNP component segregates asymmetrically and is required for self-renewal in Drosophila - pp685 - 693

Pierre Fichelson, Clara Moch, Kenzo Ivanovitch, Charlotte Martin, Clara M Sidor, Jean-Antoine Lepesant, Yohanns Bellaiche & Jean-René Huynh

doi:10.1038/ncb1874

It is unclear how the growth rate and size of asymmetrically dividing stem cells are regulated. The U3snoRNP component Wicked, required for pre-ribosomal RNA maturation and thus ribosome formation, is localized asymmetrically and is critical for stem cell maintenance and function.

Abstract - | Full Text - Live-imaging of single stem cells within their niche reveals that a U3snoRNP component segregates asymmetrically and is required for self-renewal in Drosophila | PDF (3,168 KB) - Live-imaging of single stem cells within their niche reveals that a U3snoRNP component segregates asymmetrically and is required for self-renewal in Drosophila | Supplementary information

See also: News and Views by Neumüller & Knoblich

p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug - pp694 - 704

Shu-Ping Wang, Wen-Lung Wang, Yih-Leong Chang, Chen-Tu Wu, Yu-Chih Chao, Shih-Han Kao, Ang Yuan, Chung-Wu Lin, Shuenn-Chen Yang, Wing-Kai Chan, Ker-Chau Li, Tse-Ming Hong & Pan-Chyr Yang

doi:10.1038/ncb1875

The tumour suppressor p53 inhibits cancer cell proliferation and induces apoptosis. A new tumour-suppressive effect of p53 is to induce MDM2-dependent degradation of the tumour invasion factor Slug, resulting in reduced metastasis.

Abstract - | Full Text - p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug | PDF (6,409 KB) - p53 controls cancer cell invasion by inducing the MDM2-mediated degradation of Slug | Supplementary information

A functional screen implicates microRNA-138-dependent regulation of the depalmitoylation enzyme APT1 in dendritic spine morphogenesis - pp705 - 716

Gabriele Siegel, Gregor Obernosterer, Roberto Fiore, Martin Oehmen, Silvia Bicker, Mette Christensen, Sharof Khudayberdiev, Philipp F. Leuschner, Clara J. L. Busch, Christina Kane, Katja Hübel, Frank Dekker, Christian Hedberg, Balamurugan Rengarajan, Carsten Drepper, Herbert Waldmann, Sakari Kauppinen, Michael E. Greenberg, Andreas Draguhn, Marc Rehmsmeier, Javier Martinez & Gerhard M. Schratt

doi:10.1038/ncb1876

Mir-138, identified in a screen for microRNAs associated with synapses, regulates dendritic spine morphogenesis through APT-1, a depalmitoylation enzyme that modulates the membrane localization of the heterotrimeric G protein alpha subunit.

Abstract - | Full Text - A functional screen implicates microRNA-138-dependent regulation of the depalmitoylation enzyme APT1 in dendritic spine morphogenesis | PDF (3,827 KB) - A functional screen implicates microRNA-138-dependent regulation of the depalmitoylation enzyme APT1 in dendritic spine morphogenesis | Supplementary information

See also: News and Views by Carrel & Firestein

The mitotic kinesin-14 Ncd drives directional microtubule–microtubule sliding - pp717 - 723

Gero Fink, Lukasz Hajdo, Krzysztof J. Skowronek, Cordula Reuther, Andrzej A. Kasprzak & Stefan Diez

doi:10.1038/ncb1877

Kinesin-14 (Ncd in Drosophila, and Klp2 in fission yeast) is a microtubule-based motor important for spindle assembly. Ncd and Klp2 mediate both sliding of anti-parallel microtubules and crosslinking of parallel ones.

First Paragraph - | Full Text - The mitotic kinesin-14 Ncd drives directional microtubule–microtubule sliding | PDF (3,892 KB) - The mitotic kinesin-14 Ncd drives directional microtubule–microtubule sliding | Supplementary information

See also: Letter by Braun et al.

The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism - pp724 - 730

Marcus Braun, Douglas R. Drummond, Robert A. Cross & Andrew D. McAinsh

doi:10.1038/ncb1878

Kinesin-14 (Ncd in Drosophila, and Klp2 in fission yeast) is a microtubule-based motor important for spindle assembly. Ncd and Klp2 mediate both sliding of anti-parallel microtubules and crosslinking of parallel ones.

First Paragraph - | Full Text - The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism | PDF (3,739 KB) - The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism | Supplementary information

See also: Letter by Fink et al.

Local auxin biosynthesis modulates gradient-directed planar polarity in Arabidopsis - pp731 - 738

Yoshihisa Ikeda, Shuzhen Men, Urs Fischer, Anna N. Stepanova, José M. Alonso, Karin Ljung & Markus Grebe

doi:10.1038/ncb1879

Arabidopsis root-hair position and orientation in epithelial cells is directed by an auxin gradient maximum at the root tip. The Raf-like kinase CTR1 negatively regulates local auxin biosynthesis in the root to determine root hair position.

First Paragraph - | Full Text - Local auxin biosynthesis modulates gradient-directed planar polarity in Arabidopsis | PDF (2,258 KB) - Local auxin biosynthesis modulates gradient-directed planar polarity in Arabidopsis | Supplementary information

The Patched dependence receptor triggers apoptosis through a DRAL–caspase-9 complex - pp739 - 746

Frédéric Mille, Chantal Thibert, Joanna Fombonne, Nicolas Rama, Catherine Guix, Hideki Hayashi, Véronique Corset, John C. Reed & Patrick Mehlen

doi:10.1038/ncb1880

Shh acts as a survival factor and in its absence its receptor, Patched, induces cell death by recruiting a caspase-activating complex formed by the adaptor protein DRAL, the CARD domain containing proteins TUCAN or NALP1, and caspase-9.

First Paragraph - | Full Text - The Patched dependence receptor triggers apoptosis through a DRAL–caspase-9 complex | PDF (1,531 KB) - The Patched dependence receptor triggers apoptosis through a DRAL–caspase-9 complex | Supplementary information

The bioenergetic and antioxidant status of neurons is controlled by continuous degradation of a key glycolytic enzyme by APC/C–Cdh1 - pp747 - 752

Angel Herrero-Mendez, Angeles Almeida, Emilio Fernández, Carolina Maestre, Salvador Moncada & Juan P. Bolaños

doi:10.1038/ncb1881

The glycolytic enzyme Pfkfb3 is shown to be constitutively ubiquitylated by the APC/C ligase and degraded in neurons. These findings might explain the lower glycolytic metabolism in these cells relative to astrocytes.

First Paragraph - | Full Text - The bioenergetic and antioxidant status of neurons is controlled by continuous degradation of a key glycolytic enzyme by APC/C–Cdh1 | PDF (4,955 KB) - The bioenergetic and antioxidant status of neurons is controlled by continuous degradation of a key glycolytic enzyme by APC/C–Cdh1 | Supplementary information

Replication stress induces sister-chromatid bridging at fragile site loci in mitosis - pp753 - 760

Kok Lung Chan, Timea Palmai-Pallag, Songmin Ying & Ian D. Hickson

doi:10.1038/ncb1882

The FANC and the BLM pathways collaborate during mitosis to prevent micronucleation and chromosome abnormalities.

First Paragraph - | Full Text - Replication stress induces sister-chromatid bridging at fragile site loci in mitosis | PDF (2,858 KB) - Replication stress induces sister-chromatid bridging at fragile site loci in mitosis | Supplementary information

See also: Letter by Naim & Rosselli

The FANC pathway and BLM collaborate during mitosis to prevent micro-nucleation and chromosome abnormalities - pp761 - 768

Valeria Naim & Filippo Rosselli

doi:10.1038/ncb1883

The FANC and the BLM pathways collaborate during mitosis to prevent micronucleation and chromosome abnormalities.

First Paragraph - | Full Text - The FANC pathway and BLM collaborate during mitosis to prevent micro-nucleation and chromosome abnormalities | PDF (2,428 KB) - The FANC pathway and BLM collaborate during mitosis to prevent micro-nucleation and chromosome abnormalities | Supplementary information

See also: Letter by Chan et al.

Deficiency of MIP/MTMR14 phosphatase induces a muscle disorder by disrupting Ca²⁺ homeostasis - pp769 - 776

Jinhua Shen, Wen-Mei Yu, Marco Brotto, Joseph A. Scherman, Caiying Guo, Christopher Stoddard, Thomas M. Nosek, Héctor H. Valdivia & Cheng-Kui Qu

doi:10.1038/ncb1884

Mutations in the MIP phosphatase MTMR14 are associated with human autosomal centronuclear myopathy. Mice that lack MIP have impaired muscle performance and enhanced fatigue due to the accumulation of MIP substrates PtdIns(3,5)P2 and PtdIns(3,4)P2, which cause alterations in intracellular Ca2+ levels.

First Paragraph - | Full Text - Deficiency of MIP/MTMR14 phosphatase induces a muscle disorder by disrupting Ca2+ homeostasis | PDF (1,472 KB) - Deficiency of MIP/MTMR14 phosphatase induces a muscle disorder by disrupting Ca2+ homeostasis | Supplementary information

TGF- signalling is regulated by Schnurri-2-dependent nuclear translocation of CLIC4 and consequent stabilization of phospho-Smad2 and 3 - pp777 - 784

Anjali Shukla, Mariam Malik, Christophe Cataisson, Yan Ho, Travis Friesen, Kwang S. Suh & Stuart H. Yuspa

doi:10.1038/ncb1885

In keratinocytes, the multifunctional protein CLIC4 mediates TGF--dependent growth inhibition through interaction with Schnurri-2, which is essential for CLIC4 nuclear translocation. Once in the nucleus, CLIC4 associates with phospho-Smad2 and 3 and protects them from dephosphorylation by nuclear phosphatases.

First Paragraph - | Full Text - TGF- signalling is regulated by Schnurri-2-dependent nuclear translocation of CLIC4 and consequent stabilization of phospho-Smad2 and 3 | PDF (1,637 KB) - TGF- signalling is regulated by Schnurri-2-dependent nuclear translocation of CLIC4 and consequent stabilization of phospho-Smad2 and 3 | Supplementary information