Letter abstract

Nature Cell Biology 11, 631 - 636 (2009)
Published online: 12 April 2009 | doi:10.1038/ncb1869

Production of offspring from a germline stem cell line derived from neonatal ovaries

Kang Zou1, Zhe Yuan1, Zhaojuan Yang1, Huacheng Luo1, Kejing Sun1, Li Zhou1, Jie Xiang1, Lingjun Shi1, Qingsheng Yu1, Yong Zhang1, Ruoyu Hou1 & Ji Wu1


The idea that females of most mammalian species have lost the capacity for oocyte production at birth1, 2, 3, 4, 5 has been challenged recently by the finding that juvenile and adult mouse ovaries possess mitotically active germ cells6. However, the existence of female germline stem cells (FGSCs) in postnatal mammalian ovaries still remains a controversial issue among reproductive biologists and stem cell researchers6, 7, 8, 9, 10. We have now established a neonatal mouse FGSC line, with normal karyotype and high telomerase activity, by immunomagnetic isolation and culture for more than 15 months. FGSCs from adult mice were isolated and cultured for more than 6 months. These FGSCs were infected with GFP virus and transplanted into ovaries of infertile mice. Transplanted cells underwent oogenesis and the mice produced offspring that had the GFP transgene. These findings contribute to basic research into oogenesis and stem cell self-renewal and open up new possibilities for use of FGSCs in biotechnology and medicine.

  1. School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.

Correspondence to: Ji Wu1 e-mail: jiwu@sjtu.edu.cn


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