Abstract
In Saccharomyces cerevisiae1 and HeLa cells2, the NoCut checkpoint, which involves the chromosome passenger kinase Aurora B, delays the completion of cytokinesis in response to anaphase defects. However, how NoCut monitors anaphase progression has not been clear. Here, we show that retention of chromatin in the plane of cleavage is sufficient to trigger NoCut, provided that Aurora/Ipl1 localizes properly to the spindle midzone, and that the ADA histone acetyltransferase complex is intact. Furthermore, forcing Aurora onto chromatin was sufficient to activate NoCut independently of anaphase defects. These findings provide the first evidence that NoCut is triggered by the interaction of acetylated chromatin with the passenger complex at the spindle midzone.
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Acknowledgements
We are grateful to Patrick Steigemann, Daniel Gerlich, Patrick Meraldi, Hemmo Meyer and all members of the Barral lab for fruitful discussions and critical reading of the manuscript. Thanks to Dominik Theler, Trinidad Sanmartin and Joelle Sasse for technical assistance, Orna Cohen-Fix for sharing reagents, and the ETH Light Microscopy Center for their invaluable support. This work was supported by an SNF Grant to Y.B. (2-77542-04).
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Mendoza, M., Norden, C., Durrer, K. et al. A mechanism for chromosome segregation sensing by the NoCut checkpoint. Nat Cell Biol 11, 477–483 (2009). https://doi.org/10.1038/ncb1855
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DOI: https://doi.org/10.1038/ncb1855
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