Letter abstract
Nature Cell Biology 11, 1332 - 1339 (2009)
Published online: 25 October 2009 | doi:10.1038/ncb1977
Intraflagellar transport is required for polarized recycling of the TCR/CD3 complex to the immune synapse
Francesca Finetti1, Silvia Rossi Paccani1, Maria Giovanna Riparbelli1, Emiliana Giacomello2, Giuseppe Perinetti3, Gregory J. Pazour4, Joel L. Rosenbaum5 & Cosima T. Baldari1
Most eukaryotic cells have a primary cilium which functions as a sensory organelle1. Cilia are assembled by intraflagellar transport (IFT), a process mediated by multimeric IFT particles and molecular motors2. Here we show that lymphoid and myeloid cells, which lack primary cilia, express IFT proteins. IFT20, an IFT component essential for ciliary assembly3, 4, was found to colocalize with both the microtubule organizing centre (MTOC) and Golgi and post-Golgi compartments in T-lymphocytes. In antigen-specific conjugates, IFT20 translocated to the immune synapse. IFT20 knockdown resulted in impaired T-cell receptor/CD3 (TCR/CD3) clustering and signalling at the immune synapse, due to defective polarized recycling. Moreover, IFT20 was required for the inducible assembly of a complex with other IFT components (IFT57 and IFT88) and the TCR. The results identify IFT20 as a new regulator of immune synapse assembly in T cells and provide the first evidence to implicate IFT in membrane trafficking in cells lacking primary cilia, thereby introducing a new perspective on IFT function beyond its role in ciliogenesis.
- Department of Evolutionary Biology, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.
- Department of Neuroscience, University of Siena, Via Aldo Moro 2, 53100 Siena, Italy.
- Department of Biomedicine, University of Trieste, 34138 Trieste, Italy.
- Program in Molecular Medicine, University of Massachussetts Medical School, Worcester, MA 01605, USA.
- Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
Correspondence to: Cosima T. Baldari1 e-mail: baldari@unisi.it
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