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Letter

Nature Cell Biology 10, 866–873 (1 July 2008) | doi:10.1038/ncb1747

Nitric oxide-induced nuclear GAPDH activates p300/CBP and mediates apoptosis

Nilkantha Sen , Makoto R. Hara , Michael D. Kornberg , Matthew B. Cascio , Byoung-Il Bae , Neelam Shahani , Bobby Thomas , Ted M. Dawson , Valina L. Dawson , Solomon H. Snyder & Akira Sawa

Besides its role in glycolysis, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) initiates a cell death cascade. Diverse apoptotic stimuli activate inducible nitric oxide synthase (iNOS) or neuronal NOS (nNOS), with the generated nitric oxide (NO) S-nitrosylating GAPDH, abolishing its catalytic activity and conferring on it the ability to bind to Siah1, an E3-ubiquitin-ligase with a nuclear localization signal (NLS).