Article abstract

Nature Cell Biology 10, 538 - 546 (2008)
Published online: 13 April 2008 | doi:10.1038/ncb1716

RNF4 is a poly-SUMO-specific E3 ubiquitin ligase required for arsenic-induced PML degradation

Michael H. Tatham1,3, Marie-Claude Geoffroy1,3, Linnan Shen1,3, Anna Plechanovova1, Neil Hattersley1, Ellis G. Jaffray1, Jorma J. Palvimo2 & Ronald T. Hay1

In acute promyelocytic leukaemia (APL), the promyelocytic leukaemia (PML) protein is fused to the retinoic acid receptor alpha (RAR). This disease can be treated effectively with arsenic, which induces PML modification by small ubiquitin-like modifiers (SUMO) and proteasomal degradation. Here we demonstrate that the RING-domain-containing ubiquitin E3 ligase, RNF4 (also known as SNURF), targets poly-SUMO-modified proteins for degradation mediated by ubiquitin. RNF4 depletion or proteasome inhibition led to accumulation of mixed, polyubiquitinated, poly-SUMO chains. PML protein accumulated in RNF4-depleted cells and was ubiquitinated by RNF4 in a SUMO-dependent fashion in vitro. In the absence of RNF4, arsenic failed to induce degradation of PML and SUMO-modified PML accumulated in the nucleus. These results demonstrate that poly-SUMO chains can act as discrete signals from mono-SUMOylation, in this case targeting a poly-SUMOylated substrate for ubiquitin-mediated proteolysis.

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  1. Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK.
  2. Department of Medical Biochemistry, University of Kuopio, P.O. Box 1627, FI-70211, Kuopio, Finland.
  3. These authors made an equal contribution to the work.

Correspondence to: Ronald T. Hay1 e-mail: r.t.hay@dundee.ac.uk



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