Letter abstract
Nature Cell Biology 10, 1463 - 1469 (2008)
Published online: 23 November 2008 | doi:10.1038/ncb1806
Activation of TGF-
/activin signalling resets the circadian clock through rapid induction of Dec1 transcripts
Naohiro Kon1, Tsuyoshi Hirota1, Takeshi Kawamoto2, Yukio Kato2, Tadashi Tsubota1 & Yoshitaka Fukada1
The circadian clock is reset by external time cues for synchronization to environmental changes1. In mammals, the light-input signalling pathway mediated by Per gene induction has been extensively studied2, 3. On the other hand, little is known about resetting mechanisms that are independent of Per induction4, 5, 6. Here we show that activation of activin receptor-like kinase (ALK), triggered by TGF-
, activin or alkali signals, evoked resetting of the cellular clock independently of Per induction. The resetting was mediated by an immediate-early induction of Dec1, a gene whose physiological role in the function of the circadian clock has been unclear. Acute Dec1 induction was a prerequisite for ALK-mediated resetting and upregulation was dependent on SMAD3, which was phosphorylated for activation in response to the resetting stimuli. Intraperitoneal injection of TGF- into wild-type or Dec1-deficient mice demonstrated that Dec1 has an essential role in phase-shift of clock gene expression in the kidney and adrenal gland. These results indicate that ALK–SMAD3–Dec1 signalling provides an input pathway in the mammalian molecular clock.
- Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, Japan.
- Department of Dental and Medical Biochemistry, Hiroshima University Graduate School of Biomedical Sciences, Hiroshima 734-8553, Japan.
Correspondence to: Yoshitaka Fukada1 e-mail: sfukada@mail.ecc.u-tokyo.ac.jp
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