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Letter

Nature Cell Biology 10, 1224–1231 (1 October 2008) | doi:10.1038/ncb1783

A functional RNAi screen links O-GlcNAc modification of ribosomal proteins to stress granule and processing body assembly

Takbum Ohn , Nancy Kedersha , Tyler Hickman , Sarah Tisdale & Paul Anderson

Stress granules (SGs) and processing bodies (PBs) are microscopically visible ribonucleoprotein granules that cooperatively regulate the translation and decay of messenger RNA. Using an RNA-mediated interference-based screen, we identify 101 human genes required for SG assembly, 39 genes required for PB assembly, and 31 genes required for coordinate SG and PB assembly.