Article abstract
Nature Cell Biology 1, 88 - 93 (1999)
Published online: 15 May 1999 | doi:10.1038/10054
Centrosome duplication in mammalian somatic cells requires E2F and Cdk2–Cyclin A
Patrick Meraldi1, Jiri Lukas2, Andrew M. Fry1,3, Jiri Bartek2 & Erich A. Nigg1
Abstract
Centrosome duplication is a key requirement for bipolar spindle formation and correct segregation of chromosomes during cell division. In a manner highly reminiscent of DNA replication, the centrosome must be duplicated once, and only once, in each cell cycle. How centrosome duplication is regulated and coordinated with other cell-cycle functions remains poorly understood. Here, we have established a centrosome duplication assay using mammalian somatic cells. We show that centrosome duplication requires the activation of E2F transcription factors and Cdk2–cyclin A activity.
- Department of Molecular Biology, University of Geneva , 30 quai Ernest-Ansermet, CH-1211 Geneva 4, Switzerland
- Danish Cancer Society, Institute of Cancer Biology, Department of Cell Cycle and Cancer, Strandboulevarden 49 , DK-2100 Copenhagen Ø , Denmark
- Department of Biochemistry, Adrian Building, University of Leicester, University Road, Leicester LE1 7RH, UK
Correspondence to: Erich A. Nigg1 e-mail: erich.nigg@molbio.unige.ch
