Browse Articles

  • News & Views |

    Exosomes are heterogeneous, nanoscale vesicles that mediate cellular communication. A study now leverages a size separation strategy to identify sub-classes of nanoparticles, revealing a subtype without an encapsulating membrane and variation in vesicle cargo, suggesting that size is not the only driver of heterogeneity.

    • Andries Zijlstra
    •  & Dolores Di Vizio
  • News & Views |

    Cytoplasmic flows are essential for various cellular processes. However, tools to manipulate these flows within cells are still lacking. Now research shows that an optical tool allows for control of cytoplasmic flows and can be used as a subcellular rheometer.

    • Karsten Kruse
    • , Nicolas Chiaruttini
    •  & Aurélien Roux
  • News & Views |

    N6-methyladenosine (m6A) mRNA modification influences mRNA fate by stimulating recruitment of m6A reader proteins. A previously unappreciated class of m6A reader proteins is now shown to use a common RNA-binding domain and flanking regions to selectively bind m6A-containing mRNAs, increasing their translation and stability.

    • Katherine I. Zhou
    •  & Tao Pan
  • Review Article |

    In this Review Article, Klionsky and co-authors discuss selective autophagy pathways that degrade unwanted cytosolic components and organelles, and how these pathways require ligand receptors and scaffold proteins for cargo specificity.

    • Damián Gatica
    • , Vikramjit Lahiri
    •  & Daniel J. Klionsky
  • Review Article |

    Autophagy and cancer: In this Review, Galluzzi and colleagues discuss the cellular and molecular mechanisms whereby autophagy functions in multiple aspects of malignant disease, including cancer initiation, progression and responses to therapy.

    • Marissa D. Rybstein
    • , José Manuel Bravo-San Pedro
    • , Guido Kroemer
    •  & Lorenzo Galluzzi
  • News & Views |

    Inflammation and reactive oxygen species (ROS) production after central nervous system injury are thought to enhance tissue damage and hamper neuronal regeneration. Evidence now suggests that NADPH2 oxidase delivery from macrophages to injured neurons through extracellular vesicles, promotes ROS signalling and axon recovery.

    • Eva-Maria Krämer-Albers
  • Editorial |

    Autophagy is a cellular degradation and recycling process with complex roles in health and disease and emerging relevance to translational research. In this issue, we launch a Series of commissioned articles that will discuss recent advances and outstanding questions driving this rapidly expanding and diverse field.

  • Article |

    Huang et al. identify IGF2BPs as an additional class of N6-methyladenosine (m6A) reader proteins. They find that IGF2BPs selectively bind to m6A-containing mRNAs and promote their stability.

    • Huilin Huang
    • , Hengyou Weng
    • , Wenju Sun
    • , Xi Qin
    • , Hailing Shi
    • , Huizhe Wu
    • , Boxuan Simen Zhao
    • , Ana Mesquita
    • , Chang Liu
    • , Celvie L. Yuan
    • , Yueh-Chiang Hu
    • , Stefan Hüttelmaier
    • , Jennifer R. Skibbe
    • , Rui Su
    • , Xiaolan Deng
    • , Lei Dong
    • , Miao Sun
    • , Chenying Li
    • , Sigrid Nachtergaele
    • , Yungui Wang
    • , Chao Hu
    • , Kyle Ferchen
    • , Kenneth D. Greis
    • , Xi Jiang
    • , Minjie Wei
    • , Lianghu Qu
    • , Jun-Lin Guan
    • , Chuan He
    • , Jianhua Yang
    •  & Jianjun Chen
  • Review Article |

    Mechanical forces influence both cytoplasmic and nuclear events. Kirby and Lammerding discuss recent evidence suggesting that the nucleus itself is a mechanosensor and methods to study nuclear mechanotransduction.

    • Tyler J. Kirby
    •  & Jan Lammerding
  • Article |

    Hawk et al. show that RIPK1 activation during extracellular matrix detachment induces mitophagy through mitochondrial phosphatase PGAM5 to increase reactive oxygen species and non-apoptotic cell death, and that antagonizing RIPK1/PGAM5 enhances tumour formation.

    • Mark A. Hawk
    • , Cassandra L. Gorsuch
    • , Patrick Fagan
    • , Chan Lee
    • , Sung Eun Kim
    • , Jens C. Hamann
    • , Joshua A. Mason
    • , Kelsey J. Weigel
    • , Matyas Abel Tsegaye
    • , Luqun Shen
    • , Sydney Shuff
    • , Junjun Zuo
    • , Stephan Hu
    • , Lei Jiang
    • , Sarah Chapman
    • , W. Matthew Leevy
    • , Ralph J. DeBerardinis
    • , Michael Overholtzer
    •  & Zachary T. Schafer
  • Resource |

    Lyden and colleagues use asymmetric flow field-flow fractionation to classify nanoparticles derived from cell lines and human samples, including previously uncharacterized large, Exo-L and small, Exo-S, exosome subsets.

    • Haiying Zhang
    • , Daniela Freitas
    • , Han Sang Kim
    • , Kristina Fabijanic
    • , Zhong Li
    • , Haiyan Chen
    • , Milica Tesic Mark
    • , Henrik Molina
    • , Alberto Benito Martin
    • , Linda Bojmar
    • , Justin Fang
    • , Sham Rampersaud
    • , Ayuko Hoshino
    • , Irina Matei
    • , Candia M. Kenific
    • , Miho Nakajima
    • , Anders Peter Mutvei
    • , Pasquale Sansone
    • , Weston Buehring
    • , Huajuan Wang
    • , Juan Pablo Jimenez
    • , Leona Cohen-Gould
    • , Navid Paknejad
    • , Matthew Brendel
    • , Katia Manova-Todorova
    • , Ana Magalhães
    • , José Alexandre Ferreira
    • , Hugo Osório
    • , André M. Silva
    • , Ashish Massey
    • , Juan R. Cubillos-Ruiz
    • , Giuseppe Galletti
    • , Paraskevi Giannakakou
    • , Ana Maria Cuervo
    • , John Blenis
    • , Robert Schwartz
    • , Mary Sue Brady
    • , Héctor Peinado
    • , Jacqueline Bromberg
    • , Hiroshi Matsui
    • , Celso A. Reis
    •  & David Lyden
  • Article |

    Hervera et al. show that extracellular vesicles containing NOX2 complexes are released from macrophages and incorporated into injured axons, leading to axonal regeneration through PI3K–p-Akt signalling.

    • Arnau Hervera
    • , Francesco De Virgiliis
    • , Ilaria Palmisano
    • , Luming Zhou
    • , Elena Tantardini
    • , Guiping Kong
    • , Thomas Hutson
    • , Matt C. Danzi
    • , Rotem Ben-Tov Perry
    • , Celio X. C. Santos
    • , Alexander N. Kapustin
    • , Roland A. Fleck
    • , José Antonio Del Río
    • , Thomas Carroll
    • , Vance Lemmon
    • , John L. Bixby
    • , Ajay M. Shah
    • , Mike Fainzilber
    •  & Simone Di Giovanni
  • Article |

    Xie and colleagues find that activated mTORC1 growth signalling impairs DNA repair through S6K-mediated phosphorylation and inhibition of the RNF168 ligase.

    • Xiaoduo Xie
    • , Hongli Hu
    • , Xinyuan Tong
    • , Long Li
    • , Xiangyuan Liu
    • , Min Chen
    • , Huairui Yuan
    • , Xia Xie
    • , Qingrun Li
    • , Yuxue Zhang
    • , Huafang Ouyang
    • , Mengqi Wei
    • , Jing Huang
    • , Pengda Liu
    • , Wenjian Gan
    • , Yong Liu
    • , Anyong Xie
    • , Xiaoling Kuai
    • , Gung-Wei Chirn
    • , Hu Zhou
    • , Rong Zeng
    • , Ronggui Hu
    • , Jun Qin
    • , Fei-Long Meng
    • , Wenyi Wei
    • , Hongbin Ji
    •  & Daming Gao
  • Article |

    Zajac et al. show that in colorectal cancer, decreased TGF-β signalling promotes apical actomyosin contractility and collective apical budding of invading tumour spheres with inverted polarity that drive metastatic spread.

    • Olivier Zajac
    • , Joel Raingeaud
    • , Fotine Libanje
    • , Celine Lefebvre
    • , Dora Sabino
    • , Isabelle Martins
    • , Pétronille Roy
    • , Clara Benatar
    • , Charlotte Canet-Jourdan
    • , Paula Azorin
    • , Mélanie Polrot
    • , Patrick Gonin
    • , Salima Benbarche
    • , Sylvie Souquere
    • , Gerard Pierron
    • , Damien Nowak
    • , Ludovic Bigot
    • , Michel Ducreux
    • , David Malka
    • , Camille Lobry
    • , Jean-Yves Scoazec
    • , Clarisse Eveno
    • , Marc Pocard
    • , Jean-Luc Perfettini
    • , Dominique Elias
    • , Peggy Dartigues
    • , Diane Goéré
    •  & Fanny Jaulin
  • Technical Report |

    Mittasch et al. show that controlling cytoplasmic flow via focused-light-induced cytoplasmic streaming (FLUCS), a non-invasive technique, can be used to invert asymmetric cell division in Caenorhabditis elegans zygotes.

    • Matthäus Mittasch
    • , Peter Gross
    • , Michael Nestler
    • , Anatol W. Fritsch
    • , Christiane Iserman
    • , Mrityunjoy Kar
    • , Matthias Munder
    • , Axel Voigt
    • , Simon Alberti
    • , Stephan W. Grill
    •  & Moritz Kreysing
  • Article |

    van Haren et al. develop a tool to rapidly dissociate proteins from the growing end of microtubules through photo-induced disassembly of end-binding protein 1 (EB1), and find that this reduces microtubule growth and alters cell migration.

    • Jeffrey van Haren
    • , Rabab A. Charafeddine
    • , Andreas Ettinger
    • , Hui Wang
    • , Klaus M. Hahn
    •  & Torsten Wittmann
  • News & Views |

    Mitochondria contain their own circular mitochondrial DNA (mtDNA), which, over time, is subjected to mutations that may eventually result in functional defects. A study now describes genetic and metabolic selection processes during germ cell development that prevent accumulation of harmful mtDNA variants.

    • Di Chen
    •  & Amander T. Clark
  • Editorial |

    A strong relationship between mentor and mentee is a crucial part of the training and professional development of scientists. Here, we discuss the means of successful mentorship and debunk some myths surrounding mentoring.