Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation

Latest Research

  • Review Article |

    Mechanical forces influence both cytoplasmic and nuclear events. Kirby and Lammerding discuss recent evidence suggesting that the nucleus itself is a mechanosensor and methods to study nuclear mechanotransduction.

    • Tyler J. Kirby
    •  & Jan Lammerding
  • Article |

    Hawk et al. show that RIPK1 activation during extracellular matrix detachment induces mitophagy through mitochondrial phosphatase PGAM5 to increase reactive oxygen species and non-apoptotic cell death, and that antagonizing RIPK1/PGAM5 enhances tumour formation.

    • Mark A. Hawk
    • , Cassandra L. Gorsuch
    • , Patrick Fagan
    • , Chan Lee
    • , Sung Eun Kim
    • , Jens C. Hamann
    • , Joshua A. Mason
    • , Kelsey J. Weigel
    • , Matyas Abel Tsegaye
    • , Luqun Shen
    • , Sydney Shuff
    • , Junjun Zuo
    • , Stephan Hu
    • , Lei Jiang
    • , Sarah Chapman
    • , W. Matthew Leevy
    • , Ralph J. DeBerardinis
    • , Michael Overholtzer
    •  & Zachary T. Schafer
  • Resource |

    Lyden and colleagues use asymmetric flow field-flow fractionation to classify nanoparticles derived from cell lines and human samples, including previously uncharacterized large, Exo-L and small, Exo-S, exosome subsets.

    • Haiying Zhang
    • , Daniela Freitas
    • , Han Sang Kim
    • , Kristina Fabijanic
    • , Zhong Li
    • , Haiyan Chen
    • , Milica Tesic Mark
    • , Henrik Molina
    • , Alberto Benito Martin
    • , Linda Bojmar
    • , Justin Fang
    • , Sham Rampersaud
    • , Ayuko Hoshino
    • , Irina Matei
    • , Candia M. Kenific
    • , Miho Nakajima
    • , Anders Peter Mutvei
    • , Pasquale Sansone
    • , Weston Buehring
    • , Huajuan Wang
    • , Juan Pablo Jimenez
    • , Leona Cohen-Gould
    • , Navid Paknejad
    • , Matthew Brendel
    • , Katia Manova-Todorova
    • , Ana Magalhães
    • , José Alexandre Ferreira
    • , Hugo Osório
    • , André M. Silva
    • , Ashish Massey
    • , Juan R. Cubillos-Ruiz
    • , Giuseppe Galletti
    • , Paraskevi Giannakakou
    • , Ana Maria Cuervo
    • , John Blenis
    • , Robert Schwartz
    • , Mary Sue Brady
    • , Héctor Peinado
    • , Jacqueline Bromberg
    • , Hiroshi Matsui
    • , Celso A. Reis
    •  & David Lyden
  • Article |

    Hervera et al. show that extracellular vesicles containing NOX2 complexes are released from macrophages and incorporated into injured axons, leading to axonal regeneration through PI3K–p-Akt signalling.

    • Arnau Hervera
    • , Francesco De Virgiliis
    • , Ilaria Palmisano
    • , Luming Zhou
    • , Elena Tantardini
    • , Guiping Kong
    • , Thomas Hutson
    • , Matt C. Danzi
    • , Rotem Ben-Tov Perry
    • , Celio X. C. Santos
    • , Alexander N. Kapustin
    • , Roland A. Fleck
    • , José Antonio Del Río
    • , Thomas Carroll
    • , Vance Lemmon
    • , John L. Bixby
    • , Ajay M. Shah
    • , Mike Fainzilber
    •  & Simone Di Giovanni
  • Article |

    Xie and colleagues find that activated mTORC1 growth signalling impairs DNA repair through S6K-mediated phosphorylation and inhibition of the RNF168 ligase.

    • Xiaoduo Xie
    • , Hongli Hu
    • , Xinyuan Tong
    • , Long Li
    • , Xiangyuan Liu
    • , Min Chen
    • , Huairui Yuan
    • , Xia Xie
    • , Qingrun Li
    • , Yuxue Zhang
    • , Huafang Ouyang
    • , Mengqi Wei
    • , Jing Huang
    • , Pengda Liu
    • , Wenjian Gan
    • , Yong Liu
    • , Anyong Xie
    • , Xiaoling Kuai
    • , Gung-Wei Chirn
    • , Hu Zhou
    • , Rong Zeng
    • , Ronggui Hu
    • , Jun Qin
    • , Fei-Long Meng
    • , Wenyi Wei
    • , Hongbin Ji
    •  & Daming Gao

News & Comment

  • News & Views |

    Mitochondria contain their own circular mitochondrial DNA (mtDNA), which, over time, is subjected to mutations that may eventually result in functional defects. A study now describes genetic and metabolic selection processes during germ cell development that prevent accumulation of harmful mtDNA variants.

    • Di Chen
    •  & Amander T. Clark
  • Editorial |

    A strong relationship between mentor and mentee is a crucial part of the training and professional development of scientists. Here, we discuss the means of successful mentorship and debunk some myths surrounding mentoring.

  • News & Views |

    Intrinsic factors that regulate dormancy of disseminated tumour cells (DTCs) are predominantly unknown. Now, knockdown of MSK1 is shown to accelerate bone metastasis of luminal breast cancer cells. MSK1 acts through epigenetic mechanisms that enforce the luminal phenotype and promote steady-state maintenance of DTCs within bone.

    • Candice Alexandra Grzelak
    •  & Cyrus Michael Ghajar
  • News & Views |

    Elucidating regulation of mitochondrial mRNA translation will improve our understanding of mitochondrial function during homeostasis and disease. Ribonuclease EXD2 is now shown to be important for mitochondrial translation and oxidative phosphorylation, and its loss enhances ROS production, slows development and extends lifespan in flies.

    • Brian M. Zid
    •  & Pankaj Kapahi
  • News & Views |

    Selective autophagy is important for controlled degradation of cellular components. However, a selective autophagic degradation mechanism for ribosomes in mammals has remained unclear. A study now describes non-selective and selective ribosome degradation and a significant role for ‘bystander’ non-selective autophagy.

    • Christian Münch
    •  & Ivan Dikic
  • News & Views |

    The unfolded protein response (UPR) regulates cell metabolism and survival in response to stress, yet how the UPR is connected to other signalling pathways is poorly understood. PERK is now shown to regulate Bmal1 and Clock proteins to promote cancer cell survival, revealing a link between growth regulation and circadian rhythms.

    • Miguel Sanchez-Alvarez
    •  & Chris Bakal

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