Benedetta Artegiani and Delilah Hendriks, Hubrecht Institute, Utrecht, The Netherlands

Fast and efficient generation of knock-in human organoids using homology-independent CRISPR–Cas9 precision genome editing

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    The lack of endogenous reporter lines is a bottleneck in the study of subcellular dynamics in human adult stem cell (ASC)-derived organoids. An approach using CRISPR–Cas9-mediated homology-independent organoid transgenesis (CRISPR–HOT) in ASC-derived organoids now narrows the gap between basic research and translational studies in human organoids.

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