RESEARCH ARTICLES
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RESEARCH ARTICLES |
February 1998 Table of Contents |
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February 1998 Volume 16 Number 2 p181 Delivery of antigen-encoding plasmid DNA into the cytosol of macrophages by attenuated suicide Listeria monocytogenes Guido Dietrich1,6, Andreas Bubert1,6, Ivaylo Gentschev1, Zeljka Sokolovic1, Andreas Simm2, Andre Catic3, Stefan H.E. Kaufmann3,4, Jürgen Hess3,4, Aladar A. Szalay1,5, and Werner Goebel1,* 1Lehrstuhl für Mikrobiologie and 2Department of Physiological Chemistry, University of Würzburg, D-97074 Würzburg, Germany. 3Department of Immunology, University of Ulm, D-89070 Ulm, Germany. 4Max-Planck-Institute for Infection Biology, D-10117 Berlin. 5School of Medicine, Center for Molecular Biology and Gene Therapy, Loma Linda University, Loma Linda, CA 92350. 6Both of these authors contributed equally to this work. *Corresponding author (e-mail: goebel@biozentrum.uni-wuerzburg.de). Eukaryotic expression vectors can be delivered to macrophages using attenuated self-destructing Listeria monocytogenes. L. monocytogenes cells are preferentially lysed in the host cell macrophage cytosol by the production of a PactA-dependent Listeria-specific phage lysin. Efficient expression of the cloned reporter genes by the macrophages and subsequent antigen presentation were achieved after the delivery of eukaryotic expression vectors by the attenuated suicide L. monocytogenes strain. After delivery by L. monocytogenes plasmid DNAs were found to integrate into the macrophage cell's genome at a frequency of about 107. |
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