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Volume 29 Issue 1, January 2011

The mixture of maternal and paternal sets of chromosomes in diploid organisms makes it difficult to determine haplotypes. Fan et al. and Kitzman et al. describe experimental approaches to genome-wide haplotyping using microfluidics and multiplexed large-insert cloning, respectively. Credit: Marina Corral & Erin Dewalt, based on “Colored LM of a normal male karyotype” by L. Willatt, East Anglian Regional Genetics Service/Photo Researchers, Inc.

Volume 29 Issue 1

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Editorial

  • A European guideline on biosimilar monoclonal antibodies suggests smaller trials with homogeneous, younger patient groups may suffice for marketing authorization.

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News

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News Feature

  • As oncology drug after oncology drug fails to achieve accelerated approval, sponsors are seeking other ways to speed trials. Malorye Allison investigates.

    • Malorye Allison
    News Feature

  • With the construction of two new manufacturing plants, the bioplastics market emerges. Daniel Grushkin reports.

    • Daniel Grushkin
    News Feature
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Correspondence

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Commentary

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Patents

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News & Views

  • Experimental haplotyping of whole genomes is now feasible, enabling new studies aimed at linking sequence variation to human phenotypes and disease susceptibility.

    • Vikas Bansal
    • Ryan Tewhey
    • Nicholas J Schork
    News & Views

  • Expressing zinc-finger nucleases in zygotes enables targeted transgene integration in the mouse and rat genomes.

    • Meng Amy Li
    • Allan Bradley
    News & Views

  • Screening for safe harbor sites in the genome may improve the safety of gene therapy.

    • David A Williams
    • Adrian J Thrasher
    News & Views
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Research Highlights

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Feature

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Article

  • The two copies of each chromosome in a diploid organism may contain different patterns of genetic variants. Fan et al. describe a microfluidic device capable of isolating each of the sister chromatids from single cells, allowing whole-genome haplotyping by sequencing and arrays.

    • H Christina Fan
    • Jianbin Wang
    • Stephen R Quake
    Article
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Letter

  • Cui et al. generate transgenic rats and mice bearing targeted genomic integrations by enhancing the rate of homologous recombination in single-cell embryos with zinc-finger nucleases. The approach avoids the time-consuming backcrossing involved in generating mutant mice with ES cells and should be applicable to species for which ES cells have not been isolated.

    • Xiaoxia Cui
    • Diana Ji
    • Edward J Weinstein
    Letter

  • Song et al. present the first method for global analysis of 5-hydroxymethylcytosine, a recently identified epigenetic modification in mammalian cells. They use a bacteriophage-derived enzyme to tag the hydroxymethyl group with an azide-modified glucose residue that can be used for affinity purification and sequencing of modified genomic DNA fragments.

    • Chun-Xiao Song
    • Keith E Szulwach
    • Chuan He
    Letter

  • If transgenes are to be introduced into the genome for cell therapies, the integration events should permit high transgene expression without altering the expression of endogenous genes. Papapetrou et al. propose five criteria to define such 'safe harbors' in the human genome and demonstrate high A-globin expression from a safe-harbor site in erythroid-lineage cells derived from induced pluripotent stem (iPS) cells.

    • Eirini P Papapetrou
    • Gabsang Lee
    • Michel Sadelain
    Letter
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