Research

This list contains the 50 most recently published research articles, including advance online publication articles that have not yet been published in a journal issue.

Showing: 1–25 of 50

AOP
  1. Minimum information about a single amplified genome (MISAG) and a metagenome-assembled genome (MIMAG) of bacteria and archaea Open

    Skip authorsShow fewer authors

    Standards for sequencing the microbial 'uncultivated majority', namely bacterial and archaeal single-cell genome sequences, and genome sequences from metagenomic datasets, are proposed.

  2. A wellness study of 108 individuals using personal, dense, dynamic data clouds

    Skip authorsShow fewer authors

    Longitudinal clinical and multi-omics data from 108 healthy individuals are analyzed to identify putative biomarkers and diagnostics of early disease states.

    See also: News and Views by Atul J Butte

  3. Nanoscale imaging of clinical specimens using pathology-optimized expansion microscopy

    Skip authorsShow fewer authors

    Expansion microscopy, a technique for super-resolution imaging, is extended to clinical human tissue samples that are formalin fixed, paraffin embedded, stained and/or fresh frozen.

  4. A light- and calcium-gated transcription factor for imaging and manipulating activated neurons AOP

    Skip authorsShow fewer authors

    Activity remodels neurons, altering their molecular, structural, and electrical characteristics. To enable the selective characterization and manipulation of these neurons, we present FLARE, an engineered transcription factor that drives expression of fluorescent proteins, opsins, and other genetically encoded tools only in the subset of neurons that experienced activity during a user-defined time window. FLARE senses the coincidence of elevated cytosolic calcium and externally applied blue light, which together produce translocation of a membrane-anchored transcription factor to the nucleus to drive expression of any transgene. In cultured rat neurons, FLARE gives a light-to-dark signal ratio of 120 and a high- to low-calcium signal ratio of 10 after 10 min of stimulation. Opsin expression permitted functional manipulation of FLARE-marked neurons. In adult mice, FLARE also gave light- and motor-activity-dependent transcription in the cortex. Due to its modular design, minute-scale temporal resolution, and minimal dark-state leak, FLARE should be useful for the study of activity-dependent processes in neurons and other cells that signal with calcium.

  5. Haplotype phasing of whole human genomes using bead-based barcode partitioning in a single tube AOP

    Skip authorsShow fewer authors

    Haplotype information for whole genomes is rapidly generated with a single-tube method.

  6. Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator

    Skip authorsShow fewer authors

    Lipid production in the oleaginous microalga Nannocholoropsis gaditana is doubled by decreasing the expression of a transcriptional regulator identified through a CRISPR–Cas9 reverse-genetics approach.

    See also: News and Views by Matthew C Posewitz

  7. Computational design of trimeric influenza-neutralizing proteins targeting the hemagglutinin receptor binding site

    Skip authorsShow fewer authors

    A method to target trimeric membrane proteins by designing geometrically matched trimeric protein binders is demonstrated for the influenza-virus glycoprotein hemagglutinin.

  8. 1,003 reference genomes of bacterial and archaeal isolates expand coverage of the tree of life Open

    Skip authorsShow fewer authors

    Metagenomic and microbial sequence data are made easier to interpret with the addition of 1,003 genomes to the Genomic Encyclopedia of Bacteria and Archaea.

  9. The promise of organ and tissue preservation to transform medicine

    Skip authorsShow fewer authors

    The ability to replace organs and tissues on demand could save or improve millions of lives each year globally and create public health benefits on par with curing cancer. Unmet needs for organ and tissue preservation place enormous logistical limitations on transplantation, regenerative medicine, drug discovery, and a variety of rapidly advancing areas spanning biomedicine. A growing coalition of researchers, clinicians, advocacy organizations, academic institutions, and other stakeholders has assembled to address the unmet need for preservation advances, outlining remaining challenges and identifying areas of underinvestment and untapped opportunities. Meanwhile, recent discoveries provide proofs of principle for breakthroughs in a family of research areas surrounding biopreservation. These developments indicate that a new paradigm, integrating multiple existing preservation approaches and new technologies that have flourished in the past 10 years, could transform preservation research. Capitalizing on these opportunities will require engagement across many research areas and stakeholder groups. A coordinated effort is needed to expedite preservation advances that can transform several areas of medicine and medical science.

  10. HLA-E-expressing pluripotent stem cells escape allogeneic responses and lysis by NK cells

    Skip authorsShow fewer authors

    Expression of the minimally polymorphic HLA-E molecule prevents NK-cell-mediated rejection of cells lacking expression of HLA-A, B and C.

    See also: News and Views by Steven C Kim et al.