Latest Research

  • Article |

    Efficient Cas9 genome editing in vivo is achieved without viral vectors using chemically modified single-guide RNAs.

    • Hao Yin
    • , Chun-Qing Song
    • , Sneha Suresh
    • , Qiongqiong Wu
    • , Stephen Walsh
    • , Luke Hyunsik Rhym
    • , Esther Mintzer
    • , Mehmet Fatih Bolukbasi
    • , Lihua Julie Zhu
    • , Kevin Kauffman
    • , Haiwei Mou
    • , Alicia Oberholzer
    • , Junmei Ding
    • , Suet-Yan Kwan
    • , Roman L Bogorad
    • , Timofei Zatsepin
    • , Victor Koteliansky
    • , Scot A Wolfe
    • , Wen Xue
    • , Robert Langer
    •  & Daniel G Anderson
  • Perspective |

    Recent developments in bioengineering promise the possibility of new diagnostic and treatment strategies, novel industrial processes, and innovative approaches to thorny problems in fields such as nutrition, agriculture, and biomanufacturing. As modern genetics has matured and developed technologies of increasing power, debates over risk assessments and proper applications of the technology, and over who should have decision-making power over such issues, have become more prominent. Recently, some scientists have advocated that ethicists “step out of the way,” whereas others have called for greater ethical scrutiny, or even for moratoria on some lines of research. As a community, however, we must together determine the proper application of these powerful biological tools. This paper, a consensus statement of a group of interdisciplinary delegates drawn from the top biotech-producing countries of the world, offers a set of ethical principles to contribute to the ethical conversation about human cellular biotechnological research moving forward.

    • Paul Root Wolpe
    • , Karen S Rommelfanger
    •  & the Drafting and Reviewing Delegates of the BEINGS Working Groups
  • Letter |

    Proteomes of distinct cell types in the brains of live mice are labeled and quantitated with a genetically encoded non-canonical amino-acid labeling method and click chemistry.

    • Beatriz Alvarez-Castelao
    • , Christoph T Schanzenbächer
    • , Cyril Hanus
    • , Caspar Glock
    • , Susanne tom Dieck
    • , Aline R Dörrbaum
    • , Ina Bartnik
    • , Belquis Nassim-Assir
    • , Elena Ciirdaeva
    • , Anke Mueller
    • , Daniela C Dieterich
    • , David A Tirrell
    • , Julian D Langer
    •  & Erin M Schuman
  • Article |

    Protein origami using coiled-coil building blocks produces self-assembling polyhedral cages for diverse applications.

    • Ajasja Ljubetič
    • , Fabio Lapenta
    • , Helena Gradišar
    • , Igor Drobnak
    • , Jana Aupič
    • , Žiga Strmšek
    • , Duško Lainšček
    • , Iva Hafner-Bratkovič
    • , Andreja Majerle
    • , Nuša Krivec
    • , Mojca Benčina
    • , Tomaž Pisanski
    • , Tanja Ćirković Veličković
    • , Adam Round
    • , José María Carazo
    • , Roberto Melero
    •  & Roman Jerala
  • Analysis |

    Testing 21 different fecal DNA extraction protocols in multiple laboratories results in a standardized protocol with the potential to improve comparability across human gut microbiome studies.

    • Paul I Costea
    • , Georg Zeller
    • , Shinichi Sunagawa
    • , Eric Pelletier
    • , Adriana Alberti
    • , Florence Levenez
    • , Melanie Tramontano
    • , Marja Driessen
    • , Rajna Hercog
    • , Ferris-Elias Jung
    • , Jens Roat Kultima
    • , Matthew R Hayward
    • , Luis Pedro Coelho
    • , Emma Allen-Vercoe
    • , Laurie Bertrand
    • , Michael Blaut
    • , Jillian R M Brown
    • , Thomas Carton
    • , Stéphanie Cools-Portier
    • , Michelle Daigneault
    • , Muriel Derrien
    • , Anne Druesne
    • , Willem M de Vos
    • , B Brett Finlay
    • , Harry J Flint
    • , Francisco Guarner
    • , Masahira Hattori
    • , Hans Heilig
    • , Ruth Ann Luna
    • , Johan van Hylckama Vlieg
    • , Jana Junick
    • , Ingeborg Klymiuk
    • , Philippe Langella
    • , Emmanuelle Le Chatelier
    • , Volker Mai
    • , Chaysavanh Manichanh
    • , Jennifer C Martin
    • , Clémentine Mery
    • , Hidetoshi Morita
    • , Paul W O'Toole
    • , Céline Orvain
    • , Kiran Raosaheb Patil
    • , John Penders
    • , Søren Persson
    • , Nicolas Pons
    • , Milena Popova
    • , Anne Salonen
    • , Delphine Saulnier
    • , Karen P Scott
    • , Bhagirath Singh
    • , Kathleen Slezak
    • , Patrick Veiga
    • , James Versalovic
    • , Liping Zhao
    • , Erwin G Zoetendal
    • , S Dusko Ehrlich
    • , Joel Dore
    •  & Peer Bork
  • Analysis |

    The Microbiome Quality Control project consortium reports outcomes of a baseline study (MBQC) that will guide future improvements in reproducibility of microbiome analyses.

    • Rashmi Sinha
    • , Galeb Abu-Ali
    • , Emily Vogtmann
    • , Anthony A Fodor
    • , Boyu Ren
    • , Amnon Amir
    • , Emma Schwager
    • , Jonathan Crabtree
    • , Siyuan Ma
    • , The Microbiome Quality Control Project Consortium
    • , Christian C Abnet
    • , Rob Knight
    • , Owen White
    •  & Curtis Huttenhower
  • Article | | open

    Draft genome, 994 re-sequenced lines and GWAS for yield-traits provide a resource of genetics and genomics tools for pearl millet researchers and breeders.

    • Rajeev K Varshney
    • , Chengcheng Shi
    • , Mahendar Thudi
    • , Cedric Mariac
    • , Jason Wallace
    • , Peng Qi
    • , He Zhang
    • , Yusheng Zhao
    • , Xiyin Wang
    • , Abhishek Rathore
    • , Rakesh K Srivastava
    • , Annapurna Chitikineni
    • , Guangyi Fan
    • , Prasad Bajaj
    • , Somashekhar Punnuri
    • , S K Gupta
    • , Hao Wang
    • , Yong Jiang
    • , Marie Couderc
    • , Mohan A V S K Katta
    • , Dev R Paudel
    • , K D Mungra
    • , Wenbin Chen
    • , Karen R Harris-Shultz
    • , Vanika Garg
    • , Neetin Desai
    • , Dadakhalandar Doddamani
    • , Ndjido Ardo Kane
    • , Joann A Conner
    • , Arindam Ghatak
    • , Palak Chaturvedi
    • , Sabarinath Subramaniam
    • , Om Parkash Yadav
    • , Cécile Berthouly-Salazar
    • , Falalou Hamidou
    • , Jianping Wang
    • , Xinming Liang
    • , Jérémy Clotault
    • , Hari D Upadhyaya
    • , Philippe Cubry
    • , Bénédicte Rhoné
    • , Mame Codou Gueye
    • , Ramanjulu Sunkar
    • , Christian Dupuy
    • , Francesca Sparvoli
    • , Shifeng Cheng
    • , R S Mahala
    • , Bharat Singh
    • , Rattan S Yadav
    • , Eric Lyons
    • , Swapan K Datta
    • , C Tom Hash
    • , Katrien M Devos
    • , Edward Buckler
    • , Jeffrey L Bennetzen
    • , Andrew H Paterson
    • , Peggy Ozias-Akins
    • , Stefania Grando
    • , Jun Wang
    • , Trilochan Mohapatra
    • , Wolfram Weckwerth
    • , Jochen C Reif
    • , Xin Liu
    • , Yves Vigouroux
    •  & Xun Xu
  • Review |

    The promises and potential pitfalls of shotgun metagenomics, from experimental design to computational analyses, are reviewed.

    • Christopher Quince
    • , Alan W Walker
    • , Jared T Simpson
    • , Nicholas J Loman
    •  & Nicola Segata
  • Letter |

    An in-droplet immunoassay enables determination of the rate and affinity of secreted antigen-specific antibodies at the single-cell level.

    • Klaus Eyer
    • , Raphaël C L Doineau
    • , Carlos E Castrillon
    • , Luis Briseño-Roa
    • , Vera Menrath
    • , Guillaume Mottet
    • , Patrick England
    • , Alexei Godina
    • , Elodie Brient-Litzler
    • , Clément Nizak
    • , Allan Jensen
    • , Andrew D Griffiths
    • , Jérôme Bibette
    • , Pierre Bruhns
    •  & Jean Baudry
  • Article |

    New computational tools reveal the contribution of microsatellite insertions and deletions to the mutational landscape in human cancer.

    • Yosef E Maruvka
    • , Kent W Mouw
    • , Rosa Karlic
    • , Prasanna Parasuraman
    • , Atanas Kamburov
    • , Paz Polak
    • , Nicholas J Haradhvala
    • , Julian M Hess
    • , Esther Rheinbay
    • , Yehuda Brody
    • , Amnon Koren
    • , Lior Z Braunstein
    • , Alan D'Andrea
    • , Michael S Lawrence
    • , Adam Bass
    • , Andre Bernards
    • , Franziska Michor
    •  & Gad Getz
  • Brief Communication |

    High-throughput quantification of protein and gene expression in single cells is achieved using DNA-barcoded antibodies.

    • Vanessa M Peterson
    • , Kelvin Xi Zhang
    • , Namit Kumar
    • , Jerelyn Wong
    • , Lixia Li
    • , Douglas C Wilson
    • , Renee Moore
    • , Terrill K McClanahan
    • , Svetlana Sadekova
    •  & Joel A Klappenbach
  • Resource |

    An atlas of microRNA expression patterns and regulators is produced by deep sequencing of short RNAs in human and mouse cells.

    • Derek de Rie
    • , Imad Abugessaisa
    • , Tanvir Alam
    • , Erik Arner
    • , Peter Arner
    • , Haitham Ashoor
    • , Gaby Åström
    • , Magda Babina
    • , Nicolas Bertin
    • , A Maxwell Burroughs
    • , Ailsa J Carlisle
    • , Carsten O Daub
    • , Michael Detmar
    • , Ruslan Deviatiiarov
    • , Alexandre Fort
    • , Claudia Gebhard
    • , Daniel Goldowitz
    • , Sven Guhl
    • , Thomas J Ha
    • , Jayson Harshbarger
    • , Akira Hasegawa
    • , Kosuke Hashimoto
    • , Meenhard Herlyn
    • , Peter Heutink
    • , Kelly J Hitchens
    • , Chung Chau Hon
    • , Edward Huang
    • , Yuri Ishizu
    • , Chieko Kai
    • , Takeya Kasukawa
    • , Peter Klinken
    • , Timo Lassmann
    • , Charles-Henri Lecellier
    • , Weonju Lee
    • , Marina Lizio
    • , Vsevolod Makeev
    • , Anthony Mathelier
    • , Yulia A Medvedeva
    • , Niklas Mejhert
    • , Christopher J Mungall
    • , Shohei Noma
    • , Mitsuhiro Ohshima
    • , Mariko Okada-Hatakeyama
    • , Helena Persson
    • , Patrizia Rizzu
    • , Filip Roudnicky
    • , Pål Sætrom
    • , Hiroki Sato
    • , Jessica Severin
    • , Jay W Shin
    • , Rolf K Swoboda
    • , Hiroshi Tarui
    • , Hiroo Toyoda
    • , Kristoffer Vitting-Seerup
    • , Louise Winteringham
    • , Yoko Yamaguchi
    • , Kayoko Yasuzawa
    • , Misako Yoneda
    • , Noriko Yumoto
    • , Susan Zabierowski
    • , Peter G Zhang
    • , Christine A Wells
    • , Kim M Summers
    • , Hideya Kawaji
    • , Albin Sandelin
    • , Michael Rehli
    • , The FANTOM Consortium
    • , Yoshihide Hayashizaki
    • , Piero Carninci
    • , Alistair R R Forrest
    •  & Michiel J L de Hoon
  • Resource |

    B-cell VH region repertoire sequencing of eight anatomical sites in six human donors reveals distinct networks of clone distribution.

    • Wenzhao Meng
    • , Bochao Zhang
    • , Gregory W Schwartz
    • , Aaron M Rosenfeld
    • , Daqiu Ren
    • , Joseph J C Thome
    • , Dustin J Carpenter
    • , Nobuhide Matsuoka
    • , Harvey Lerner
    • , Amy L Friedman
    • , Tomer Granot
    • , Donna L Farber
    • , Mark J Shlomchik
    • , Uri Hershberg
    •  & Eline T Luning Prak