Advance online publication
The latest research papers, published online ahead of print. These online versions are definitive and may be cited using the digital object identifier (DOI).
About advance online publicationResearch
Analysis
Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasma
Terri A Addona, Susan E Abbatiello, Birgit Schilling, Steven J Skates, D R Mani, David M Bunk, Clifford H Spiegelman, Lisa J Zimmerman, Amy-Joan L Ham, Hasmik Keshishian, Steven C Hall, Simon Allen, Ronald K Blackman, Christoph H Borchers, Charles Buck, Helene L Cardasis, Michael P Cusack, Nathan G Dodder, Bradford W Gibson, Jason M Held, Tara Hiltke, Angela Jackson, Eric B Johansen, Christopher R Kinsinger, Jing Li, Mehdi Mesri, Thomas A Neubert, Richard K Niles, Trenton C Pulsipher, David Ransohoff, Henry Rodriguez, Paul A Rudnick, Derek Smith, David L Tabb, Tony J Tegeler, Asokan M Variyath, Lorenzo J Vega-Montoto, Åsa Wahlander, Sofia Waldemarson, Mu Wang, Jeffrey R Whiteaker, Lei Zhao, N Leigh Anderson, Susan J Fisher, Daniel C Liebler, Amanda G Paulovich, Fred E Regnier, Paul Tempst & Steven A Carr
Published online: 28 June 2009 | doi:10.1038/nbt.1546
Although multiple reaction monitoring (MRM) mass spectrometry holds considerable promise for quantifying candidate protein biomarkers in blood, transferability of MRM assays between laboratories has never been shown. Addona et al. assess the reproducibility, dynamic range and limits of detection and quantification of MRM across multiple sites.
Abstract - Multi-site assessment of the precision and reproducibility of multiple reaction monitoring-based measurements of proteins in plasma | Full Text - Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasma | PDF (646 KB) - Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasma | Supplementary information
Article
Sequential treatment of drug-resistant tumors with targeted minicells containing siRNA or a cytotoxic drug
Jennifer A MacDiarmid, Nancy B Amaro-Mugridge, Jocelyn Madrid-Weiss, Ilya Sedliarou, Stefanie Wetzel, Kartini Kochar, Vatsala N Brahmbhatt, Leo Phillips, Scott T Pattison, Carlotta Petti, Bruce Stillman, Robert M Graham & Himanshu Brahmbhatt
Published online: 28 June 2009 | doi:10.1038/nbt.1547
Drug resistance remains a major hurdle to effective cancer chemotherapy. MacDiarmid et al. show that bacterially derived minicells packaged with siRNAs reverse tumor drug resistance and that subsequent treatment with minicells loaded with cytotoxic drugs causes tumor stabilization or regression.
Abstract - Sequential treatment of drug-resistant tumors with targeted minicells containing siRNA or a cytotoxic drug | Full Text - Sequential treatment of drug-resistant tumors with targeted minicells containing siRNA or a cytotoxic drug | PDF (801 KB) - Sequential treatment of drug-resistant tumors with targeted minicells containing siRNA or a cytotoxic drug | Supplementary information
Letter
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins
Debashish Ray, Hilal Kazan, Esther T Chan, Lourdes Peña Castillo, Sidharth Chaudhry, Shaheynoor Talukder, Benjamin J Blencowe, Quaid Morris & Timothy R Hughes
Published online: 28 June 2009 | doi:10.1038/nbt.1550
Until now, determining the sequences recognized by an RNA-binding protein has been time and labor intensive. Ray et al. use a custom pool of >210,000 oligos that encode linear and stem-loop RNAs to rapidly determine the sequences bound by nine RNA-binding proteins.
First Paragraph - Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins | Full Text - Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins | PDF (403 KB) - Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins | Supplementary information
Until print versions of AOP papers are published, they should be cited in the style "Author(s) Nature Biotechnology advance online publication, day month year (doi:10.1038/nbtXXXXX)". Once the print version (identical to the AOP) is published, it should be cited as follows: "Author(s) Nature Biotechnology volume, page (year); advance online publication, (doi:10.1038/nbtXXXXX)".
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