Bio/Technology
9, 648 - 651 (1991)
doi:10.1038/nbt0791-648
Real Time Automated Simultaneous Double-Stranded DNA Sequencing Using Two-Color Fluorophore LabelingHideki Kambara1, Keiichi Nagai1
& Satoko Hayasaka1
1Central Research Laboratory, Hitachi Ltd., Kokubunji, Tokyo 185, Japan. We determined the base sequences of both strands of duplex DNA simultaneously with an automated DNA sequencer. Primers for each strand were labeled with two different dyes and chain extension reactions for both strands produced four DNA families: "A", "C", "G", and "T". An image splitting-prism and band-pass filters were mounted on a real time DNA sequencer to detect fluorescences from the two different dyes separately. As a result, one thousand bases of a clone could be determined at the same time.
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