Somatic Embryogenesis in Hybrid Tea Roses

We report the regeneration of flowering plants in rose (Rosa hybrida L.) via somatic embryos continuously produced from long−term cultures of friable embryogenic tissue. Filament explants from young flower buds were cultured on a medium based on B5 modified salts giving rise to a semi−hard type callus. Globular embryos differentiated from this primary callus in the presence of 2,4−D and zeatin after subculture. These embryos are an excellent source of friable embryogenic tissue, in the presence of −napthaleneacetic acid (NAA) and zeatin, that maintains its regeneration capacity for more than 18 months through periodic subculture in a high 2,4−D/zeatin medium. Somatic embryos were periodically isolated from embryogenic tissue and have undergone differentiation, maturation, germination and plantlet development. Plantlets obtained through this method developed normally in the greenhouse, similar to the micropropagated controls. Upon flowering, these plants proved to be morphologically true to type and displayed the flower color of the donor genotype.

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