Bio/Technology
8, 547 - 549 (1990)
doi:10.1038/nbt0690-547
A New Method for Protein Crystallization Using High PressureK. Visuri1, E. Kaipainen2, J. Kivimäki2, H. Niemi2, M. Leisola1, *
& S. Palosaari2
1Cultor Ltd., Technology Center, 02460 Kantvik, Finland.
2Lappeenranta University of Technology. Department of Chemical Technology, P.O. Box 20, 53851 Lappeenranta, Finland.
*Corresponding author. Crystallization methods for proteins have been the subject of decades of development yet protein crystallization remains the limiting step in structural studies. We present here a new method for protein crystallization—based on the use of high pressure—that enabled us to accelerate dramatically the growth of glucose isomerase crystals. We think this method may have a more general utility.
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