Bio/Technology
8, 934 - 938 (1990)
doi:10.1038/nbt1090-934
Rattlesnake and Scorpion Antivenoms from the Egg Yolks of Immunized HensBruce S. Thalley1
& Sean B. Carroll1, *
1Laboratory of Molecular Biology, University of Wisconsin-Madison, 1525 Linden Drive, Madison, WI 53706.
*Corresponding author. Antivenoms used to treat poisonous bites and stings are usually derived from horse sera. Consequently, they contain horse immunoglobulins, which frequently cause complement mediated side effects, and other proteins that can cause serum sickness and, occasionally, anaphylactic shock. Here we describe a new, avian source of antivenoms that precludes these complications, and an efficient and gentle means for preparing antivenoms composed solely of venom-specific antibodies. We demonstrate that antivenoms purified from the egg yolks of laying hens immunized with Crotalus atrox rattlesnake venom and Leiurus quinquestriatus hebraeus scorpion venom neutralize the lethal effects of these venoms in vivo. Antivenoms purified from chicken eggs may be phar-maceutically safer and more economical to produce than current horse antivenoms.
REFERENCES
- Habermehl, G.G. 1981. Venomous Animals and Their Toxins. Springer-Verlag, Berlin.
- Bücherl, W. 1968. Venomous Animals and Their Venoms. W. Bücherl, E. E. Buckley, V. Deulofeu (Ed.). Academic Press, New York.
- Behring, E. von Kitasato, S. 1890. Über das Zustandekommen der Diphtheriae Immunität und der Tetanus-Immunität bei Thieren. Dtsch. med. Wschr. 16: 1113−1114.
- Phisalix, C., Bertrand, G. 1894. Sur la propriété antitoxique du sang des animaux vaccinés contre le venin de vipère. C. R. Acad. Sci., Paris. 118: 356−358.
- Calmette, A. 1894. Contribution à l'étude du venin des serpants. Immunisation des animaux et traitement de l'envenimation. Ann. Inst. Pasteur 8: 275−291.
- Roux, M.E., Martin, M.L., Chaillou, M.A. 1894. Trois cents ca
 de diphtérie traités par le sérum antidiphtérique. Ann. Inst. Pasteur Lille 8: 639−661.
- Calmette, A. 1896. The treatment of animals poisoned with snake venom by the injection of antivenomous serum. Lancet II: 449−450.
- Sullivan, J.B. 1987. Past, present, and future immunotherapy of snake venom poisoning. Annals of Emerg. Med. 16: 938−944. | ISI |
- Ellenhorn, M.J., Barceloux, D.G. 1988. Envenomation from bites and stings, p. 1112−1132. In: Medical Toxicology: Diagnosis and Treatment of Human Poisoning. Elsevier, New York.
- Sutherland, S.K. 1977. Serum Reactions. An analysis of commercial antivenoms and the possible role of anticomplementary activity in denovo reactions to antivenoms and antitoxins. Med. J. Aust. 1: 613−615. | PubMed | ISI | ChemPort |
- World Health Organization, 1981. Progress in the characterization of venoms and standardization of antivenoms. Who Offset Publication No. 58. Geneva.
- Parrish, H.M., Hayes, R.H. 1970. Hospital management of pit viper venenations. Clinical Toxicology 3(3): 501−511. | PubMed | ISI | ChemPort |
- Baxter, E.H., Marr, A.G.M. 1974. Sea Wasp (Chironex fleckeri) antivenene: neutralizing potency against the venom of three other jelly fish species. Toxicon 12: 223−229. | Article | PubMed | ISI | ChemPort |
- Cohen, P., Seligmann, Jr., E.B. 1966. Immunological studies of coral snake venom. Mem. Inst. Butanan. 33: 339−347.
- Mohamed, A.H., Bakr, I.A., Kamel, A. 1966. Egyptian polyvalent antisnakebite serum; technic of preparation. Toxicon 4: 69−72. | Article | PubMed | ISI | ChemPort |
- Russell, F.E., Timmerman, W.F., Meadows, P.E. 1970. Clinical use of antivenin prepared from goat serum. Toxicon 8: 63−65. | Article | PubMed | ISI | ChemPort |
- Kocholaty, W.F., Bowles-Ledford, E., Daly, J.G., Billings, T.A. 1971. Preparation of coral snake antivenin from goat serum. Toxicon 9: 297−298. | Article | PubMed | ISI | ChemPort |
- Benson, H.N., Brumfield, H.P., Pomeroy, B.S. 1961. Requirement of avian C1 for fixation of guinea pig complement by avian antibody-antigen complexes. J. Immunol. 87: 616−622. | PubMed | ISI | ChemPort |
- Rose, M.E., Orlans, E. 1962. Fowl antibody IV. The estimation of haemolytic fowl complement. Immunology 5: 642−648. | PubMed | ISI | ChemPort |
- Polson, A., von Wechmar, M.B., van Regenmortel, M.H.V. 1980. Isolation of viral IgY antibodies from yolks of immunized hens. Immunological Communications 9(5): 475−493. | PubMed | ISI | ChemPort |
- Carroll, S.B. 1989. U.S. Patent Application (pending).
- Clement, J.E., Pietrusko, R.G. 1979. Pit viper snake bite envenomation in the United States. Clin. Toxicol. 14: 515−538. | ISI |
- Iwanaga, S., Suzuki, T. 1979. Enzymes in snake venom, p. 61−144. In: Snake Venoms. C.Y. Lee (Ed.). Springer-Verlag, Berlin.
- Lissitzky, S., Miranda, F., Etzensperger, P., Mercier, J. 1956. Sur la toxicité du venin de deux espèces de scorpions nord-africains. Compt. Rend. Soc. Biol. 150: 741−743. | ISI | ChemPort |
- Miranda, F., Lissitzky, S. 1958. Purification de la toxine du venin de scorpion. Biochim. Biophys. Acta. 30: 217−218. | Article | PubMed | ISI | ChemPort |
- Hassan, F. 1984. Production of scorpion antivenin, p. 577−605. In: Insect Poisons, Allergens, and other Invertebrate Venoms. A. T. Tu (Ed.). Marcel Dekker, New York.
- Benedict, A.A., Yamaga, K. 1966. Immunoglobulins and antibody production in avian species, p. 335−375. In: Comparative Immunology J.J. Marchaloni (Ed.). Blackwell, Oxford.
- Yang, C.C., Lin, M.F. 1977. Purification of anticobrotoxin antibody by affinity chromatography. Toxicon 15: 51−62. | Article | PubMed | ISI | ChemPort |
- Russell, F.E., Sullivan, J.B., Egen, N.B., Jeter, W.S., Markland, F.S., Wingert, W.A., Bar-Or, D. 1985. Preparation of a new antivenin by affinity chromatography. Am. Soc. Trop. Med. and Hygiene 34(1): 141−150. | ChemPort |
- Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265−275. | PubMed | ISI | ChemPort |
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