Journal home
Advance online publication
Current issue
Archive
Press releases
Supplements
Focuses
Conferences
Guide to authors
Online submissionOnline submission
Permissions
For referees
Free online issue
Contact the journal
Subscribe
Advertising
work@npg
naturereprints
About this site
For librarians
 
NPG Resources
Bioentrepreneur
Nature Reviews Drug Discovery
Nature
Nature Medicine
Nature Genetics
Nature Reviews Genetics
Nature Methods
Nature Chemical Biology
news@nature.com
Clinical Pharmacology & Therapeutics
Nature Conferences
NPG Subject areas
Biotechnology
Cancer
Chemistry
Clinical Medicine
Dentistry
Development
Drug Discovery
Earth Sciences
Evolution & Ecology
Genetics
Immunology
Materials Science
Medical Research
Microbiology
Molecular Cell Biology
Neuroscience
Pharmacology
Physics
Browse all publications
Research Papers
Bio/Technology  7, 811 - 816 (1989)
doi:10.1038/nbt0889-811

Transgenic Tobacco Plants Expressing a Bacterial Detoxifying Enzyme are Resistant to 2,4-D

Wolfgang R. Streber1, 2, * & Lothar Willmitzer2

  1Schering AG Pflanzenschutzforschung, Müllerstrasse 178, D-1000 Berlin 65, FRG.

  2Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000 Berlin 33, FRG.

  *Corresponding author.

We have used a gene from the soil bacterium Alcaligenes eutrophus JMP134 to introduce a herbicide degrading mechanism into plants. The gene, tfdA, which encodes a 2,4-dichlorophenoxyacetate monooxygenase (DPAM) catalyzing the first step in the bacterial 2,4-D degradative pathway, has recently been cloned and sequenced. The coding sequence, fused with plant expression sequences was introduced into plants via Agrobacterium-mediated gene transfer. The chimeric gene was expressed in tobacco (Nicotiana tabacum) under the control of either a constitutive or a light-inducible promoter. Transgenic tobacco lines tolerated elevated levels of 2,4-D in tissue culture, and regenerated plants showed resistance when sprayed with the herbicide.

REFERENCES
  1. Botterman, J. and Leemans, J. 1988. Engineering herbicide resistance in plants. Trends in Genetics 4:219−222. | PubMed  | ISI | ChemPort |
  2. Cheung, A.Y., Bogorad, L., Van Montagu, M. and Schell, J. 1988. Relocating a gene for herbicide tolerance: A chloroplast gene is converted into a nuclear gene. Proc. Natl. Acad. Sci. USA 85:391−395. | ChemPort |
  3. Comai, L., Facciotti, D., Hiatt, W.R., Thompson, G., Rose, R.E. and Stalker, D.M. 1985. Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317:741−744. | ISI | ChemPort |
  4. Haughn, G.W., Smith, J., Mazur, B. and Somerville, C. 1988. Transformation with a mutant Arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides. Mol. Gen. Genet. 211:266−271. | ISI | ChemPort |
  5. DeBlock, M., Bottermann, J., Vandewiele, M., Dockx, J., Thoen, C., Gosselé, V., Rao Movva, N., Thompson, C., Van Montagu, M. and Leemans, J. 1987. Engineering herbizide resistance in plants by expression of a detoxyfying enzyme. EMBO J. 6:2513−2518. | ISI | ChemPort |
  6. Stalker, D.M., McBride, K.E. and Malyj, L.D. 1988. Herbicide resistance in transgenic plants expressing a bacterial detoxification gene. Science 242:419−423. | ISI | ChemPort |
  7. Estelle, M.A. and Somerville, C. 1987. Auxin-resistant mutants of Arabidopsis thaliana with an altered morphology. Mol. Gen. Genet. 206:200−206. | ISI | ChemPort |
  8. Zenk, M.H. 1974. Haploids in physiological and biochemical research, In: Haploids in Higher Plants. Advances and potential. Proceedings of the First International Symposium, Guelph, Ontario, Canada.
  9. Streber, W.S., Timmis, K.N. and Zenk, M.H. 1987. Analysis, cloning, and high-level expression of 2,4-dichlorophenoxyacetate monooxygenase gene tfdA of Alcaligenes eutrophus JMP134. J. Bacteriol. 169:2950−2955. | PubMed  | ISI | ChemPort |
  10. Lütcke, H.A., Chow, K.C., Mickel, F.S., Moss, K.A., Kern, H.F. and Scheele, G.A. 1987. Selection of AUG initiation codons differs in plants and animals. EMBO J. 6:43−48. | PubMed  | ISI | ChemPort |
  11. Dhaese, P., De Greve, H., Gielen, J., Seurinck, J., Van Montagu, M., Schell, J. 1983. Identification of sequences involved in the polyadenylation of higher plant nuclear transcripts using Agrobacterium T-DNA genes as models. EMBO J. 2:419−426. | PubMed  | ISI | ChemPort |
  12. Paszkowski, J., Shillito, R.D., Saul, M., Mandák, V., Hohn, T., Hohn, B. and Potrykus, I. 1984. Direct gene transfer to plants. EMBO J. 3:2717−2722. | ISI | ChemPort |
  13. Pietrzak, M., Shillito, R.D., Hohn, T. and Potrykus, I. 1986. Expression in plants of two bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector. Nucl. Acids Res. 14:5857−5868. | PubMed  | ISI | ChemPort |
  14. Jones, J.D., Dunsmuir, P. and Bedbrook, J. 1985. High level expression of introduced chimaeric genes in regenerated transformed plants. EMBO J. 4:2411−2418. | ISI | ChemPort |
  15. Stockhaus, J., Eckes, P., Rocha-Sosa, M., Schell, J. and Willmitzer, L. 1987. Analysis of cis-active sequences involved in the leaf-specific expression of a potato gene in transgenic plants. Proc. Natl. Acad. Sci. USA 84:7943−7947. | ChemPort |
  16. Perkins, E.J., Stiff, C.M. and Lurquin, P.F. 1987. Use of Alcaligenes eutrophus as a source of genes for 2,4-D resistance in plants. Weed Science 35:12−18. | ISI | ChemPort |
  17. Fraley, R.T., Rogers, S.G., Horsch, R.B., Sanders, P.R., Flick, J.S., Adams, S.P., Bittner, M.L., Brand, L.A., Fink, C.L., Fry, J.S., Galluppi, G.R., Goldberg, S.B., Hoffmann, N.L. and Woo, S.C. 1983. Expression of bacterial genes in plant cells. Proc. Natl. Acad. Sci. USA 80:4803−4807. | PubMed  | ChemPort |
  18. Swanson, E.B. and Tomes, D.T. 1983. Evaluation of birdsfoot trefoil regenerated plants and their progeny after in vitro selection for 2,4-dichlorophenoxyacetic acid tolerance. Plant Science Letters 29:19−24. | Article | ISI | ChemPort |
  19. Yanisch-Perron, C., Vieira, J. and Messing, J. 1985. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103−119. | Article | PubMed  | ChemPort |
  20. Van Haute, E., Joos, H., Maes, M., Warren, G., Van Montagu, M. and Schell, J. 1983. Intergenic transfer and exchange recombinations of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti-plasmid of Agrobacterium tumefaciens. EMBO J. 2:411−417. | PubMed  | ChemPort |
  21. Norrander, J., Kempe, T. and Messing, J. 1983. Construction of improved M13 vectors using oligonucleotide-directed mutagenesis. Gene 26:101−106. | Article | PubMed  | ISI | ChemPort |
  22. Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant 15:473−497. | ISI | ChemPort |
  23. Sanchez-Serrano, J.J., Leil, M., O'Connor, A., Schell, J. and Willmitzer, L. 1987. Wound-induced expression of a potato proteinase inhibitor II gene in transgenic tobacco plants. EMBO J. 6:303−306. | ChemPort |
  24. Maniatis, T., Fritsch, E.F. and Sambrook, J. 1982. Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
  25. Sanger, F., Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463−5467. | PubMed  | ChemPort |
  26. Chen, E.U. and Seeburg, P.H. 1985. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165−170. | PubMed  | ISI | ChemPort |
  27. Dellaporta, S.L., Wood, J. and Hicks, J.B. 1983. Plant DNA mini-preparation: version II. Plant. Mol. Biol. Rep. 1:19−21. | ChemPort |
  28. Southern, E. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503−517. | PubMed  | ISI | ChemPort |
 Top
 Top
Abstract
Previous | Next
Table of contents
Download PDFDownload PDF
Send to a friendSend to a friend
Save this linkSave this link

Open Innovation Challenges

  • Corrosion Inhibitor

    • Deadline: Aug 19 2009
    • Reward: $10,000 USD

    The Seeker is looking for inhibitors of corrosion. This Challenge requires only a written descripti...

  • Efficient Chromosome Doubling: Plant Cell Division

    • Deadline: Jul 15 2009
    • Reward: $20,000 USD

    The Seeker is looking for an efficient chromosome doubling method in plants and in particular, metho...

naturejobs

References
Export citation
Export references
natureproducts

Search buyers guide:

 
Nature Biotechnology
ISSN: 1087-0156
EISSN: 1546-1696
Journal home | Advance online publication | Current issue | Archive | Press releases | Supplements | Focuses | Conferences | For authors | Online submission | Permissions | For referees | Free online issue | About the journal | Contact the journal | Subscribe | Advertising | work@npg | naturereprints | About this site | For librarians
Nature Publishing Group, publisher of Nature, and other science journals and reference works©1989 Nature Publishing Group | Privacy policy