Bio/Technology
7, 811 - 816 (1989)
doi:10.1038/nbt0889-811
Transgenic Tobacco Plants Expressing a Bacterial Detoxifying Enzyme are Resistant to 2,4-DWolfgang R. Streber1, 2, *
& Lothar Willmitzer2
1Schering AG Pflanzenschutzforschung, Müllerstrasse 178, D-1000 Berlin 65, FRG.
2Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, D-1000 Berlin 33, FRG.
*Corresponding author. We have used a gene from the soil bacterium Alcaligenes eutrophus JMP134 to introduce a herbicide degrading mechanism into plants. The gene, tfdA, which encodes a 2,4-dichlorophenoxyacetate monooxygenase (DPAM) catalyzing the first step in the bacterial 2,4-D degradative pathway, has recently been cloned and sequenced. The coding sequence, fused with plant expression sequences was introduced into plants via Agrobacterium-mediated gene transfer. The chimeric gene was expressed in tobacco (Nicotiana tabacum) under the control of either a constitutive or a light-inducible promoter. Transgenic tobacco lines tolerated elevated levels of 2,4-D in tissue culture, and regenerated plants showed resistance when sprayed with the herbicide. REFERENCES
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