Abstract
We have regenerated whole plants of rice (Oryza sativa L.) derived from protoplasts, which had been electroporated with plasmid DNA possessing a chimeric gene encoding aminoglycoside phosphotransferase II (APH(3′)II). Transformed calli were selected on the basis of tolerance to the antibiotic, G418. Several plants were regenerated possessing functional APH(3′)II activity due to the integration of intact foreign DNA into their genome.
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L¨rz, H., Baker, B., and Schell, J. 1985. Gene transfer to cereal cells mediated by protoplast transformation. Molec. Gen. Genet. 199:178–182.
Potrykus, I., Saul, M.W., Petruska, J., Paszkowski, J., and Shillito, R.D. 1985. Direct gene transfer to cells of a graminaceous monocot. Molec. Gen. Genet. 199:183–188.
Fromm, M.E., Taylor, L.P., and Walbot, V. 1986. Stable transformation of maize after gene transfer by electroporation. Nature 319:791–793.
Hauptmann, R.M., Vasil, V., Ozias-Akins, P., Tabaeizadeh, Z., Rogers, S.G., Fraley, R.T., Horsch, R.B., and Vasil, I.K. 1988. Evaluation of selectable markers for obtaining stable transformants in the gramineae. Plant Physiol. 86:602–606.
Uchimiya, H., Gushimi, T., Hashimoto, H., Harada, H., Syono, K., and Sugawara, Y. 1986. Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.). Molec. Gen. Genet. 204:204–207.
Fujimura, T., Sakurai, M., Akagi, H., Negishi, T., and Hirose, A. 1985. Regeneration of rice plants from protoplasts. Plant Tissue Culture Lett. 2:74–75.
Toriyama, K., Hinata, K., and Sasaki, T. 1986. Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theor. Appl. Genet. 73:16–19.
Yamada, Y., Yang, Z.Q., and Tang, D.T. 1986. Plant regeneration from protoplast-derived callus of rice (Oryza sativa L.). Plant Cell Rep. 4:85–88.
Abdullah, R., Cocking, E.C., and Thompson, J.A. 1986. Efficient plant regeneration from rice protoplasts through somatic embryogenesis. Bio/Technology 4:1087–1090.
Kyozuka, J., Hayashi, Y., and Shimamoto, K. 1987. High frequency plant regeneration from rice protoplasts by novel nurse culture methods. Molec. Gen. Genet. 206:408–413.
Jefferson, R.A., Kavanagh, T.A., and Bevan, M.W. 1987. GUS fusions: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. EMBO J. 6:3901–3907.
Schocher, R.J., Shillito, R.D., Saul, M.W., Paszkowski, J., and Potrykus, I., 1986. Co-transformation of unlinked foreign genes into plants by direct gene transfer. Bio/Technology 4:1093–1096.
Rhodes, C.A., Pierce, D.A., Mettler, I.J., Mascarenhas, D., and Detmer, J.J. 1988. Genetically transformed maize plants from protoplasts. Science 240:204–207.
Uchimiya, H., Hirochika, H., Hashimoto, H., Hara, A., Masuda, T., Kasumimoto, T., Harada, H., Ikeda, J.E., and Yoshioka, M. 1986. Co-expression and inheritance of foreign genes in transformants obtained by direct DNA transformation of tobacco protoplasts. Molec. Gen. Genet. 205:1–8.
Toriyama, K., and Hinata, K. 1985. Cell suspension and protoplast culture in rice. Plant Sci. 41:179–183.
Gamborg, O.L., Miller, R.A., and Ojima, K. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50:151–158.
Chu, C.C., Wang, C.C., Sun, C.S., Hsu, C., Yin, K.C., Chu, C.Y., and Bi, F.Y. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci. Sin. 18:659–668.
Schreier, P.H., Seftor, E.A., Schell, J., and Bohnert, H.J. 1985. The use of nuclear-encoded sequences to direct the light-regulated synthesis and transport of a foreign protein into plant chloroplasts. EMBO J. 4:25–32.
Rogers, S.O., and Bendich, A.J. 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummigied plant tissues. Plant Mol. Biol. 5:69–76.
Southern, E.M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503–517.
Gardner, R.C., Howarth, A.J., Hahn, P., Brown-Luedi, M., Shepherd, R.J., and Messing, J. 1981. The complete nucleotide sequence of an infectious clone of cauliflower mosaic virus by M 13mp7 shotgun sequencing. Nucleic Acids Res. 9:2871–2888.
Beck, E., Ludwig, G., Auerswald, E.A., Reiss, B., and Schaller, H. 1982. Nucleotide sequence and exact localization of the neomycin phosphotransferase gene from transposon Tn5. Gene 19:327–336.
Bevan, M., Barnes, W.M., and Chilton, M. 1983. Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Res. 1:369–385.
Morota, H., and Uchimiya, H. 1987. Stable maintenance of foreign DNA in transformed cell lines of rice (Oryza sativa L.). Jpn. J. Genet. 62:363–368.
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Toriyama, K., Arimoto, Y., Uchimiya, H. et al. Transgenic Rice Plants After Direct Gene Transfer into Protoplasts. Nat Biotechnol 6, 1072–1074 (1988). https://doi.org/10.1038/nbt0988-1072
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DOI: https://doi.org/10.1038/nbt0988-1072
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