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Article
Bio/Technology  6, 1035 - 1040 (1988)
doi:10.1038/nbt0988-1035

Automated Plant Tissue Culture for Mass Propagation

Robert Levin1, Victor Gaba1, Beni Tal1, Stanley Hirsch1, David DeNola1 & Indra K. Vasil1

1by Robert Levin, Victor Gaba, Beni Tal, Stanley Hirsch, and David DeNola of Plant Biotech Industries Ltd., Mobile Post Ashrat 25201, Israel; and Indra K. Vasil, Department of Vegetable Crops, 511 Bartram Hall West, University of Florida, Gainesville, FL 32611


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  2. Vasil, I.K. (ed.). 1986. Cell Culture and Somatic Cell Genetics of Plants, Vol. 3: Plant Regeneration and Genetic Variability. Academic Press, Orlando, Fla.
  3. Zimmerman, R.H., Griesbach, R.J., Hammerschlag, F.A., and Lawson, R.H. (eds.). 1986. Tissue Culture as a Plant Production System for Horticultural Crops. Martinus Nijhoff, Boston.
  4. Morel, G. and Martin, C. 1952. Guerison de Dahlias atteints d'une maladie a virus. Compt. Rend. Acad. Sci. Ser. D 235:1324−1325. | ChemPort |
  5. Morel, G. 1960. Producing virus-free Cymbidium. Amer. Orchid Soc. Bull. 29:495−497.
  6. Morel, G. 1965. Clonal propagation of orchids by meristem culture. Cymbidium Soc. News 20:3−11.
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  8. Vasil, I.K. and Vasil, V. 1986. Regeneration in cereal and other grass species, pp. 121−150. In: Cell Culture and Somatic Cell Genetics of Plants, Vol. 3: Plant Regeneration and Genetic Variability. Academic Press, Orlando, Fla.
  9. Vasil, I.K. Developing cell and tissue culture systems for the improvement of cereal and grass crops. J. Pl. Physiol. 128:193−218.
  10. Redenbaugh, K., Viss, P., Slade, D., and Fujii, J.A. 1987. Scale up: artificial seeds, pp. 473−493. In: Plant Tissue and Cell Culture (Green, C. E., Somers, D., Hackett, W. F., and Bies-boer, D. D., eds.). Alan R. Liss, New York.
  11. Constantine, D.R. 1986. Micropropagation in the commercial environment, pp. 175−186. In: Plant Tissue Culture and its Agricultural Applications. Withers, L. A. and Alderson, P. G. (eds.). Butterworth, London.
  12. Hussey, G. and Cooke, R.C. 1979. Homogenization as an aid in tissue culture propagation of Platycerium and Davallia. HortSci. 14:21−22.
  13. Giles, K.L. and Morgan, W.M. 1987. Industrial scale plant micropropagation. Tibtech. 5:35−39.
  14. Sluis, C.J. and Walker, K.A. 1985. Commercialization of plant tissue culture. IAPTC Newslett. 47:2−12.
  15. Anderson, W.C., Meagher, G.W. and Nelson, A.G. 1972. Cost of propagating broccoli plants through tissue culture. HortSci. 12:543−544.
  16. Donnan, A., Davidson, S.E., and Williams, C.L. 1978. Establishment of tissue-grown plants in the greenhouse environment. Proc. Fla. State Hort. Soc. 91:236−237.
  17. Hussey, G. 1983. In vitro propagation of horticultural and agricultural crops, pp. 111−138. In: Plant Biotechnology. Mantell, S. H. and Smith, H. (eds.). Cambridge Univ. Press, Cambridge.
  18. Hussey, G. 1986. Problems and prospects of in vitro propagation of herbaceous plants, pp. 69−84. In: Plant Tissue Culture and its Agricultural Applications. Withers, L. A. and Alderson, P. G. (eds.). Butterworth, London.
  19. DeBry, L. 1986. Robots in plant tissue culture. IAPTC Newslett. 49:2−22.
  20. Tisserat, B. and Vandercook, C.E. 1985. Development of an automated plant culture system. Pl. Cell Tiss. Organ Cult. 5:107−117. | ISI |
  21. Weathers, P. and Giles, K.L. 1987. Regeneration of many varieties of plants using a novel method of in vitro cell culture. In Vitro Cell. Dev. Biol. 23:69A (Abstract).
  22. Maene, L. and Debergh, P. 1985. Liquid medium additions to established tissue cultures to improve elongation and rooting in vitro. Pl. Cell Tiss. Organ Cult. 5:23−33. | ISI | ChemPort |
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