Bio/Technology
6, 822 - 825 (1988)
doi:10.1038/nbt0788-822
Antibody Fingerprinting: A Novel Method for Identifying Individual People and AnimalsAnn-Michele Francoeur1
1Miragen, Inc., P.O. Box 178691, San Diego, CA 92117. Autoantibodies that react with cellular components occur in high frequency in patients with systemic rheumatic diseases. A novel class of "autoantibodies" that react with cellular components has been identified in normal humans and other species. Unlike the disease-associated autoantibodies that are restricted in number, these human autoantibodies increase in number from birth until approximately two years after birth, and then remain constant for decades, if not lifelong. The complement of such autoantibodies present in an individual is unique, and for this reason they have been named individual-specific or IS autoantibodies. The IS autoantibodies, when physically separated, comprise an "antibody fingerprint" that can serve to identify people or animals, or their body fluids. These antibodies may also be associated with "security documents" and used for purposes of authentication.
REFERENCES
- Jeffreys, A.J., Wilson, V., and Thein, S.L.. 1985. Hypervariable "minisatellite" regions in human DNA. Nature 324:67−73.
- Towbin, H., Staehelin, T., and Gordon, J.. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350−4354. | PubMed | ChemPort |
- Tan, E.M. 1982. Autoantibodies to nuclear antigens (ANA): Their immunobiology and medicine. Adv. Immunol. 33:167−240. | PubMed | ISI | ChemPort |
- Bernstein, R.M., Bunn, C.C., Hughes, G.R.V., Francoeur, A.M. and Mathews, M.B.. 1984. Cellular protein and RNA antigens in autoimmune disease. Mol. Biol. Med. 2:105−120. | PubMed | ChemPort |
- Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 277:680−685. | ISI |
- Avrameas, S., Guilbert, B., and Dighiero, G. 1981. Natural antibodies against tubulin, actin, myoglobin, thyroglobulin, fetuin, albumin and transferrin are present in normal human sera and monoclonal immunoglobulins from multiple myeloma and Waldenstrom's macroglobulinemia may express similar antibody specificity. Ann Immunol. (Inst. Pasteur) 132C:231−236. | ChemPort |
- Grabar, P. 1975. Auto-antibodies and immunological theories: An analytical review. Clin. Immunol. and Immunopathol. 4:453−340. | Article | ISI | ChemPort |
- Francoeur, A.M., Peebles, C.L., Gompper, P.T., and Tan, E.M. 1986. Identification of Ki (Ku, p70/p80) autoantigens and analysis of anti-Ki autoantibody reactivity. J. Immunol. 136:1648−1653. | PubMed | ISI | ChemPort |
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