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Insect Tolerant Transgenic Tomato Plants

Bio/Technology volume 5, pages 807–813 (1987)Cite this article

Abstract

The structure of an insect control protein gene from Bacillus thuringiensis var. kurstaki HD–1 was determined, and truncated forms of the gene that express a functional insecticidal protein were generated. Two of these truncated genes were incorporated into a plant expression vector for Agrobacterium–mediated transformation. Transgenic tomato plants containing the chimeric genes express the insect control protein gene. Such expression confers tolerance to lepidopteran larvae on the transgenic tomato plants and their progeny. These engineered tomato plants represent a significant step to increased selectivity, specificity and efficiency in insect control.

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References

  1. Dulmage, H.T. 1981. Insecticidal activity of isolates of Bacillus thuringiensis and their potential for pest control, p. 193–222. In: Microbial Control of Pests and Plant Diseases 1970-1980. H. D. Burges (ed.). Academic Press, New York.

    Google Scholar 

  2. Angus, T.A. 1954. A bacterial toxin paralysing silkworm larvae. Nature 173: 545–546.

    Article  CAS  Google Scholar 

  3. Wilcox, D.R., Shivakuma, A.G., Melin, B.E., Miller, M.F., Benson, T.A., Schopp, C.W., Casuto, D., Gundling, G.J., Boiling, T.J., Spear, B.B. and Fox, J.L. 1986. Genetic engineering of bioinsecticides, p. 395–413. In: Protein Engineering. M. Inouye and R. Sarma (eds.). Academic Press, New York.

    Google Scholar 

  4. Bulla, L.A., Kramer, K.J. and Davidson, L.I. 1977. Characterization of the entomocidal parasporal crystal of Bacillus thuringiensis . J. Bacteriol. 130: 375–383.

    CAS  PubMed  PubMed Central  Google Scholar 

  5. Schnepf, H.E., Wong, H.C. and Whiteley, H.R. 1985. The amino acid sequence of a crystal protein from Bacillus thuringiensis deduced from the DNA base sequence. J. Biol. Chem. 260: 6264–6272.

    CAS  PubMed  Google Scholar 

  6. Shibano, Y., Yamagata, A., Nakamura, N., lizuka, T., Sugisaki, H. and Takanami, M. 1985. Nucleotide sequence coding for the insecticidal fragment of the Bacillus thuringiensis crystal protein. Gene 34: 243–251.

    Article  CAS  Google Scholar 

  7. Adang, M.J., Staver, M.J., Rochleau, T.A., Leighton, J., Barker, R.F. and Thompson, D.V. 1985. Characterized full length and truncated plasmid clones of the crystal protein of Bacillus thuringiensis subsp. kurstaki HD73 and their toxicity to Manduca sexta . Gene 36: 289–300.

    Article  CAS  Google Scholar 

  8. Thorne, L., Garduno, F., Thompson, T., Decker, D., Zounes, M., Wild, M., Walfield, A.M. and Pollock, T.J. 1986. Structural similarity between the Lepidoptera and Diptera specific insecticidal endotoxin genes of Bacillus thuringiensis subsp. kurstaki and israelensis . J. Bacteriol. 166: 801–811.

    Article  CAS  Google Scholar 

  9. Wabiko, H., Raymond, K.C. and Bulla, L.A. 1986. Bacillus thuringiensis entomocidal protoxin gene sequence and gene product analysis. DNA 5: 305–314.

    Article  CAS  Google Scholar 

  10. Hofte, H., de Greve, H., Seurinck, J., Jansens, S., Mahillon, J., Ampe, C., Vandekerckhove, J., Vanderbruggen, H., van Montagu, M., Zabeau, M. and Vaeck, M. 1986. Structural and functional analysis of a cloned delta endotoxin of Bacillus thuringiensis berliner 1715. Eur. J. Biochem. 161: 273–280.

    Article  CAS  Google Scholar 

  11. Geiser, M., Schweitzer, S. and Grimm, C. 1986. The hypervariable region in the genes coding for entomopathogenic crystal proteins of Bacillus thuringiensis: Nucleotide sequence of the kurhd1 gene of subsp. kurstaki HD1. Gene 48: 109–118.

    Article  CAS  Google Scholar 

  12. Fraley, R.T., Rogers, S.G. and Horsch, R.B. 1986. Genetic transformation in higher plants. Critical Reviews in Plant Sciences. 4: 1–46.

    Article  CAS  Google Scholar 

  13. Shah, D.M., Horsch, R.B., Klee, H.J., Kishore, G.M., Winter, J.A., Turner, N.E., Hironaka, C.M., Sanders, P.R., Gasser, C.S., Aykent, S., Siegel, N.R., Rogers, S.G. and Fraley, R.T. 1986. Engineering herbicide tolerance in plants. Science 233: 478–481.

    Article  CAS  Google Scholar 

  14. Comai, L., Facciotti, D., Hiatt, W.R., Thompson, G., Rose, R.E. and Stalker, D.M. 1985. Expression in plants of a mutant aroA gene from Salmonella typhimurium confers tolerance to glyphosate. Nature 317: 741–744.

    Article  CAS  Google Scholar 

  15. Powell Abel, P., Nelson, R.S., De, B., Hoffmann, N., Rogers, S.G., Fraley, R.T. and Beachy, R.N. 1986. Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738–743.

    Article  Google Scholar 

  16. Watrud, L.S., Perlak, E.J., Tran, M.-T., Kusano, K., Mayer, E.J., Miller-Wideman, M.A., Obukowicz, M.G., Nelson, D.R., Kreitinger, J.P. and Kaufman, R.J. 1985. Cloning of the Bacillus thuringiensis subsp. kurstaki delta endotoxin gene into Pseudomonas fluorescens: Molecular biology and ecology of an engineered microbial pesticide, p. 40–46. In: Engineered Organisms in the Environment. H. O. Halvor-son, D. Pramer and M. Rogul (eds.). American Society for Microbiology, Washington.

    Google Scholar 

  17. Obukowicz, M.G., Perlak, E.J., Kusano-Kretzmer, K., Mayer, E.J. and Watrud, L.S. 1986. Integration of the delta endotoxin gene of Bacillus thuringiensis into the chromosome of root colonizing strains of pseudomonads using Tn5 . Gene 45: 327–331.

    Article  CAS  Google Scholar 

  18. Bulla, L.A., Kramer, K.J., Cox, D.J., Jones, B.L., Davidson, L.I. and Lookhart, G.L. 1981. Purification and characterization of the entomocidal protoxin of Bacillus thuringiensis . J. Biol. Chem. 256: 3000–3004.

    CAS  PubMed  Google Scholar 

  19. Nagamatsu, Y., Itai, Y., Hatanaka, C., Funatsu, G. and Hayashi, K. 1984. A toxic fragment from the entomocidal crystal protein of Bacillus thuringiensis . Agric. Biol. Chem. 48: 611–619.

    CAS  Google Scholar 

  20. Schnepf, H.E. and Whiteley, H.R. 1985. Delineation of a toxin encoding segment of a Bacillus thuringiensis crystal protein gene. J. Biol. Chem. 260: 6273–6280.

    CAS  PubMed  Google Scholar 

  21. Sanders, P.R., Winter, J.A., Barnason, A.R., Rogers, S.G. and Fraley, R.T. 1987. Comparison of cauliflower mosaic virus 35S and nopaline synthase promoters in transgenic plants. Nucleic Acids Res. 15: 1543–1558.

    Article  CAS  Google Scholar 

  22. Fraley, R.T., Rogers, S.G., Horsch, R.B., Eichholtz, D.A., Flick, J.S., Fink, C.L., Hoffmann, N.L. and Sanders, P.R. 1985. The SEV system: A new disarmed Ti plasmid vector system for plant transformation. Bio/Technology 3: 629–635.

    CAS  Google Scholar 

  23. McCormick, S.M., Niedermeyer, J., Fry, J., Barnason, A., Horsch, R. and Fraley, R. 1986. Leaf-disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens . Plant Cell Reports 5: 81–84.

    Article  CAS  Google Scholar 

  24. Odell, J.T., Nagy, F. and Chua, N.-H. 1985. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter. Nature 313: 810–812.

    Article  CAS  Google Scholar 

  25. Bevan, M., Barnes, W.M. and Chilton, M.-D. 1983. Structure and transcription of the nopaline synthase gene region of T-DNA. Nucleic Acids Res. 11: 369–385.

    Article  CAS  Google Scholar 

  26. Luthy, P. and Ebersold, H.R. 1981. Bacillus thuringiensis delta endotoxin: Histopathology and molecular mode of action, p. 235–267. In: Pathogenesis of Invertebrate Microbial Diseases. E.W. Davidson (ed.). Allanheld, Toronto.

    Google Scholar 

  27. Davidson, R.H. and Lyon, W.F. 1987. Insect Pests of Farm, Garden and Orchard, Wiley & Sons, New York.

    Google Scholar 

  28. Jones, J.D.G., Dunsmuir, P. and Bedbrook, J. 1985. High level expression of introduced chimaeric genes in regenerated transformed plants. EMBO J. 4: 2411–2418.

    Article  CAS  Google Scholar 

  29. Maniatis, T., Fritsch, E.F. and Sambrook, J. 1982. Molecular Cloning, A Laboratory Manual, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.

    Google Scholar 

  30. Sanger, F., Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Nat. Acad. Sci. USA 74: 5463–5467.

    Article  CAS  Google Scholar 

  31. Biggin, M.D., Gibson, T.J. and Hong, G.F. 1983. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination. Proc. Nat. Acad. Sci. USA 80: 3963–3965.

    Article  CAS  Google Scholar 

  32. Messing, J. and Viera, J. 1982. A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19: 269–276.

    Article  CAS  Google Scholar 

  33. Zoller, M.J. and Smith, M. 1982. Oligonucleotide-directed mutagene-sis using M13-derived vectors: An efficient and general procedure for the production of point mutations in any fragment of DNA. Nucleic Acids Res. 10: 6487–6500.

    Article  CAS  Google Scholar 

  34. Rochester, D.E., Winter, J.A. and Shah, D.M. 1986. The structure and expression of maize genes encoding the major heat shock protein hsp70. EMBO J. 5: 451–458.

    Article  CAS  Google Scholar 

  35. Abbott, W.S. 1925. A method of computing the effectiveness of an insecticide. J. Econorn. Entomol. 18: 265–267.

    Article  CAS  Google Scholar 

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Author information

Author notes

  1. Katherine S. Bowdish

    Present address: Research Institute of Scripps Clinic, La Jolla, CA

  2. Sheila M. McCormick

    Present address: Plant Gene Expression Center, USDA-ARS, Albany, CA

  3. David A. Fischhoff: To whom correspondence should be addressed.

Authors and Affiliations

  1. Monsanto Co., 700 Chesterfield Village Parkway, St. Louis, MO, 63198

    David A. Fischhoff, Katherine S. Bowdish, Frederick J. Perlak, Pamela G. Marrone, Sheila M. McCormick, Jeanne G. Niedermeyer, Duff A. Dean, Kuniko Kusano-Kretzmer, Ernest J. Mayer, Dean E. Rochester, Stephen G. Rogers & Robert T. Fraley

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  1. David A. Fischhoff
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  2. Katherine S. Bowdish
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  3. Frederick J. Perlak
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  6. Jeanne G. Niedermeyer
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  8. Kuniko Kusano-Kretzmer
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  9. Ernest J. Mayer
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  10. Dean E. Rochester
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  11. Stephen G. Rogers
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  12. Robert T. Fraley
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Fischhoff, D., Bowdish, K., Perlak, F. et al. Insect Tolerant Transgenic Tomato Plants. Nat Biotechnol 5, 807–813 (1987). https://doi.org/10.1038/nbt0887-807

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  • DOI: https://doi.org/10.1038/nbt0887-807

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