A Bacteriophage cI857 Cassette Controls P_L Expression Vectors at Physiologic Temperatures

We have developed a cassette of the bacteriophage lambda () cI857 temperature−sensitive repressor gene from which the functional P _R promoter has been deleted. This cassette was placed into an expression vector that uses the P _L promoter for expression of the herpes simplex virus type 1 (HSV−1) glycoprotein D gene (gD−1) in E. coli. Control of the gD−1 production from this plasmid was compared to an expression vector lacking the cI857 cassette but regulated by an E. coli (cI857) lysogen. The lysogen allowed expression of the gD−1 gene at 37°C, whereas cells containing the cI857 cassette maintained repression of gD−1. Both systems were fully induced at 42°C. The ability to grow cells at 37°C while maintaining repression of transcription from P _L should be advantageous for large scale fermentations.

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