Amino Terminal Acetylation of Authentic Human Cu,Zn Superoxide Dismutase Produced in Yeast

Human Cu,Zn superoxide dismutase (HSOD) is an N−acetylated enzyme, which may be useful in the prevention of post−ischemic damage. Since HSOD expressed in E. coli is not N−acetylated we have investigated expression and N−acetylation in yeast. HSOD has been expressed at very high levels in yeast utilizing the yeast glyceraldehyde phosphate dehydrogenase promoter. It is soluble, of normal specific activity and confers low level resistance to copper and zinc. The yeast HSOD is acetylated at its N−terminus indicating that yeast cells may be useful for the production of N−acetylated proteins.

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