Bio/Technology
4, 647 - 649 (1986)
doi:10.1038/nbt0786-647
Gene Transfer into Loblolly Pine by Agrobacterium tumefaciensRonald Sederoff1, Anne-Marie Stomp2, W. Scott Chilton3
& Larry W. Moore4
1Institute of Forest Genetics and the Pacific Southwest Forest and Range Experiment Station, USDA Forest Service, P.O. Box 245, Berkeley, CA 94701
2Department of Forestry, North Carolina State University, Raleigh, NC 27695
3Department of Botany, North Carolina State University, Raleigh, NC 27695
4Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331. We have demonstrated expression of bacterial genes transferred into cells of loblolly pine (Pinus taeda L.) by Agrobacterium tumefaciens. Whereas previous surveys found pines resistant to Agrobacterium, we found two wild type strains that produce galls. Callus proliferated from these galls synthesized specific opines. These results provide strong evidence for transfer and expression of bacterial genes in pine and extend the potential of genetic engineering to the world's most important genus for fiber production.
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