Transformation of Poplar by Agrobacterium tumefaciens

The ability to regenerate plants from poplar cells cultured in vitro suggests that poplars may prove a valuable model system for the application of recombinant DNA technology to deciduous trees. We report here the transformation of a hybrid Populus trichocarpa deltoides with two strains of Agrobacterium tumefaciens, as demonstrated by phytohormone autonomous growth, opine production, and Southern blot analysis.

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