Table of contents


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Editorial

Research not fit to print p115

doi:10.1038/nbt.3488

Some biotech companies now eschew traditional publication in peer-reviewed journals. Does it matter?


FDA approves 'farmaceutical' drug from transgenic chickens pp117 - 119

Cormac Sheridan

doi:10.1038/nbt0216-117


Obama's cancer moonshot p119

doi:10.1038/nbt0216-119


Biotechs target stagnant baldness market pp120 - 121

Charles Schmidt

doi:10.1038/nbt0216-120


CRISPR patents to go on trial p121

doi:10.1038/nbt0216-121a


Bayer joins CRISPR p121

doi:10.1038/nbt0216-121b


Proteasome play C4 debuts p122

doi:10.1038/nbt0216-122a


Direct Genomics revives Helicos sequencing system for China's hospitals pp122 - 123

David Cyranoski

doi:10.1038/nbt0216-122b


New partner for Galapagos JAK drug p123

doi:10.1038/nbt0216-123


Chimera research under the spotlight pp124 - 125

Laura DeFrancesco

doi:10.1038/nbt0216-124


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Correction

Corrections p125

doi:10.1038/nbt0216-125a


Around the world in a month p125

doi:10.1038/nbt0216-125b


FDA approves three different multiple myeloma drugs in one month p126

Mark Ratner

doi:10.1038/nbt0216-126


Data Page

2015—another banner year for biotech p127

Walter Yang

doi:10.1038/nbt.3480


Drug pipeline Q4 2015 p128

Laura DeFrancesco

doi:10.1038/nbt.3484


News Feature

Fresh from the biotech pipeline—2015 pp129 - 132

Chris Morrison

doi:10.1038/nbt.3472

Novel drug approvals in the United States surpassed even 2014's unprecedented totals. The broad trends are familiar but, as Chris Morrison reports, 2015 had its share of pioneering achievements.


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Bioentrepreneur

Building a business

Preparing for crises pp133 - 136

Mark Kessel & Robert Masella

doi:10.1038/nbt.3475

How can you prepare for the day when an unexpected event morphs into a crisis, with all the potential negative repercussions for your company's reputation and financial standing?


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Opinion and Comment

Correspondence

Cas9-triggered chain ablation of cas9 as a gene drive brake pp137 - 138

Bing Wu, Liqun Luo & Xiaojing J Gao

doi:10.1038/nbt.3444


Precision and robustness of 2D-NMR for structure assessment of filgrastim biosimilars pp139 - 141

Houman Ghasriani, Derek J Hodgson, Robert G Brinson, Ian McEwen, Lucinda F Buhse, Steven Kozlowski, John P Marino, Yves Aubin & David A Keire

doi:10.1038/nbt.3474


Evolving Japanese regulations on companion diagnostics pp141 - 144

Sumimasa Nagai, Masaaki Urata, Hiroyuki Sato, Motoki Mikami, Wataru Kuga, Reiko Yanagihara, Daisei Miyamoto, Yuka Suzuki & Mayumi Shikano

doi:10.1038/nbt.3478


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Features

Patents

Persistent confusion and controversy surrounding gene patents pp145 - 147

Christi J Guerrini, Mary A Majumder & Amy L McGuire

doi:10.1038/nbt.3470

The development of patent-specific educational resources and prompt resolution of patentability rules unsettled by recent US Supreme Court decisions are urgently needed in the genomics industry.


Recent patents in computational biology p148

doi:10.1038/nbt.3487


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News and Views

Can CRISPR-Cas9 gene drives curb malaria? pp149 - 150

Luke Alphey

doi:10.1038/nbt.3473

Gene drives in mosquitoes to reduce the spread of malaria move closer, though technical and regulatory hurdles remain.


Mapping regulatory elements pp151 - 152

Yuexin Zhou & Wensheng Wei

doi:10.1038/nbt.3477

Regulatory genomic elements can now be studied in their native context using two CRISPR-based high-throughput approaches.

See also: Research by Rajagopal et al. | Research by Korkmaz et al.


Personalized nutrition through big data pp152 - 154

Daniel McDonald, Gustavo Glusman & Nathan D Price

doi:10.1038/nbt.3476

A systems model of glycemic response identifies individually tailored diets that keep blood sugar in check.


Research Highlights p154

doi:10.1038/nbt.3482


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Computational Biology

Analysis

Identifying recurrent mutations in cancer reveals widespread lineage diversity and mutational specificity pp155 - 163

Matthew T Chang, Saurabh Asthana, Sizhi Paul Gao, Byron H Lee, Jocelyn S Chapman, Cyriac Kandoth, JianJiong Gao, Nicholas D Socci, David B Solit, Adam B Olshen, Nikolaus Schultz & Barry S Taylor

doi:10.1038/nbt.3391

Detection of recurrently mutated nucleotides identifies novel cancer hotspots in an analysis of >11,000 human tumor samples.


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Research

Brief Communications

Antisense oligonucleotide–directed inhibition of nonsense-mediated mRNA decay pp164 - 166

Tomoki T Nomakuchi, Frank Rigo, Isabel Aznarez & Adrian R Krainer

doi:10.1038/nbt.3427

Antisense oligonucleotides target genetic disease via gene-specific inhibition of nonsense-mediated mRNA decay.


Articles

High-throughput mapping of regulatory DNA pp167 - 174

Nisha Rajagopal, Sharanya Srinivasan, Kameron Kooshesh, Yuchun Guo, Matthew D Edwards, Budhaditya Banerjee, Tahin Syed, Bart J M Emons, David K Gifford & Richard I Sherwood

doi:10.1038/nbt.3468

Cis-regulatory genomic regions necessary for gene expression are revealed by a high-throughput CRISPR-Cas9–based assay.

See also: News and Views by Zhou & Wei


Integration of electrophysiological recordings with single-cell RNA-seq data identifies neuronal subtypes pp175 - 183

János Fuzik, Amit Zeisel, Zoltán Máté, Daniela Calvigioni, Yuchio Yanagawa, Gábor Szabó, Sten Linnarsson & Tibor Harkany

doi:10.1038/nbt.3443

Patch-seq reveals new neuronal subtypes by combining electrophysiological and RNA-seq data on single neurons in situ.


Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 pp184 - 191

John G Doench, Nicolo Fusi, Meagan Sullender, Mudra Hegde, Emma W Vaimberg, Katherine F Donovan, Ian Smith, Zuzana Tothova, Craig Wilen, Robert Orchard, Herbert W Virgin, Jennifer Listgarten & David E Root

doi:10.1038/nbt.3437

Genome-wide sgRNA libraries based on rules for on-target activity improve results of Cas9-based screens and facilitate a further refinement of on- and off-target prediction algorithms.


Letters

Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9 pp192 - 198

Gozde Korkmaz, Rui Lopes, Alejandro P Ugalde, Ekaterina Nevedomskaya, Ruiqi Han, Ksenia Myacheva, Wilbert Zwart, Ran Elkon & Reuven Agami

doi:10.1038/nbt.3450

CRISPR-Cas9 is used for both enrichment and dropout screens of functional enhancers in human cells.

See also: News and Views by Zhou & Wei


Electrophysiological, transcriptomic and morphologic profiling of single neurons using Patch-seq pp199 - 203

Cathryn R Cadwell, Athanasia Palasantza, Xiaolong Jiang, Philipp Berens, Qiaolin Deng, Marlene Yilmaz, Jacob Reimer, Shan Shen, Matthias Bethge, Kimberley F Tolias, Rickard Sandberg & Andreas S Tolias

doi:10.1038/nbt.3445

Patch-seq reveals new neuronal subtypes by combining electrophysiological and RNA-seq data on single neurons in situ.


Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain pp204 - 209

Benjamin E Deverman, Piers L Pravdo, Bryan P Simpson, Sripriya Ravindra Kumar, Ken Y Chan, Abhik Banerjee, Wei-Li Wu, Bin Yang, Nina Huber, Sergiu P Pasca & Viviana Gradinaru

doi:10.1038/nbt.3440

AAV vectors that efficiently transduce the mouse brain after intravenous injection are generated with a CRE-dependent selection system.


Errata

Erratum: Analysis of intronic and exonic reads in RNA-seq data characterizes transcriptional and post-transcriptional regulation p210

Dimos Gaidatzis, Lukas Burger, Maria Florescu & Michael B Stadler

doi:10.1038/nbt0216-210a


Erratum: Share and share alike p210

Samuel K Sia & Matthew P Owens

doi:10.1038/nbt0216-210b


Corrigendum

Corrigendum: Increasing the efficiency of precise genome editing with CRISPR-Cas9 by inhibition of nonhomologous end joining p210

Takeshi Maruyama, Stephanie K Dougan, Matthias C Truttmann, Angelina M Bilate, Jessica R Ingram & Hidde L Ploegh

doi:10.1038/nbt0216-210c


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Careers and Recruitment

Fourth-quarter biotech job picture p211

Michael Francisco

doi:10.1038/nbt.3479


People

People p212

doi:10.1038/nbt.3486


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