Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
- Benjamin E Deverman,
- Piers L Pravdo,
- Bryan P Simpson,
- Sripriya Ravindra Kumar,
- Ken Y Chan,
- Abhik Banerjee,
- Wei-Li Wu,
- Bin Yang &
- Viviana Gradinaru
Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, California, USA.
- Nina Huber &
- Sergiu P Pasca
B.E.D. designed and performed experiments, analyzed data, prepared figures and wrote the manuscript. P.L.P., B.P.S., S.R.K., A.B. and K.Y.C. performed experiments, virus production and characterization. W.-L.W. performed tissue processing and IHC. B.Y. assisted with tissue clearing and imaging. N.H. and S.P.P. performed the experiments with human cells, analyzed the data, and prepared the associated figure and text. V.G. helped with study design and data analysis, manuscript and figure preparation and supervised the project. All authors edited and approved the manuscript.
Competing financial interests
The California Institute of Technology has filed patent applications related to this work with B.E.D, B.Y. and V.G. listed as inventors.
Supplementary Figure 1: Capsid library fragment generation and Cre-dependent capsid sequence recovery.Hover over figure to zoom
Supplementary Figure 2: The most enriched variants recovered from in vivo selections in GFAP-Cre mice.Hover over figure to zoom
Supplementary Figure 3: AAV-PHP.B transduces several interneuron cell types and endothelial cells but does not appear to transduce microglia.Hover over figure to zoom
Supplementary Figure 4: Long-term eGFP expression in the brain following gene transfer with AAV-PHP.B.Hover over figure to zoom
Supplementary Figure 5: Representative images of native GFP fluorescence and IHC for several neuron and glial cell types following transduction by AAV-PHP.B:CAG-NLS-GFP.Hover over figure to zoom
Supplementary Figure 6: AAV9, AAV-PHP.A and AAV-PHP.B transduce human iPSC-derived cortical neurons and astrocytes in dissociated cultures and intact 3D cortical cultures.Hover over figure to zoom
Supplementary Figure 7: AAV-PHP.B and AAV-PHP.A capsids localize to the brain vasculature after intravenous injection and transduce cells along the vasculature by 24 hours post-administration.Hover over figure to zoom
Video 1: Supplementary Movie 1
Video 2: Supplementary Movie 2
Video 3: Supplementary Movie 3