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Acknowledgements
The authors would like to thank M. Ghorbal for extensive testing and recommendations. This work was supported by the French Parasitology consortium ParaFrap (ANR-11-LABX0024) ERC Advanced grant (PlasmoEscape 250320).
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MacPherson, C., Scherf, A. Flexible guide-RNA design for CRISPR applications using Protospacer Workbench. Nat Biotechnol 33, 805–806 (2015). https://doi.org/10.1038/nbt.3291
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DOI: https://doi.org/10.1038/nbt.3291
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