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Editorial

Old boy biotech p781

doi:10.1038/nbt.3308

No matter where you look in biopharmaceutical boardrooms, gender bias is evident. Is it so difficult to actually acknowledge this pernicious bias and actively discourage all-male boards?


'Financial toxicity' looms as cancer combinations proliferate pp783 - 784

Chris Morrison

doi:10.1038/nbt0815-783


New class PCSK9 blockers stride into cholesterol market pp785 - 786

Cormac Sheridan

doi:10.1038/nbt0815-785


Microbial cocktails join fecal transplants in IBD treatment trials pp787 - 788

Mark Ratner

doi:10.1038/nbt0815-787


Blockbuster expectations for hepatitis B therapeutic vaccine p789

Suzanne Elvidge

doi:10.1038/nbt0815-789


NCI-MATCH pairs tumor mutations with matching drugs pp790 - 791

Vicki Brower

doi:10.1038/nbt0815-790


Alnylam, Dicerna tussle over RNAi tech p791

doi:10.1038/nbt0815-791a


Court invalidates Sequenom's prenatal test patent p791

doi:10.1038/nbt0815-791b


PureTech floats away p792

doi:10.1038/nbt0815-792a


BGI goes head-to-head with Illumina p792

doi:10.1038/nbt0815-792b


Oxitec trials GM sterile moth to combat agricultural infestations pp792 - 793

Emily Waltz

doi:10.1038/nbt0815-792c


Around the world in a month p793

doi:10.1038/nbt0815-793


Data Page

2Q15—Running of the biotech bulls p794

Walter Yang

doi:10.1038/nbt.3311


Drug pipeline: 2Q15 p795

Laura DeFrancesco

doi:10.1038/nbt.3312


News Feature

To share is human pp796 - 800

doi:10.1038/nbt.3309

Personal health data are beginning to be shared in the digital world—on mobile devices and the internet. Is the loss of privacy a price worth paying for the presumed benefits? Laura DeFrancesco investigates.


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Bioentrepreneur

Building a business

In house expertise pp801 - 804

Orin Herskowitz

doi:10.1038/nbt.3302

Executive-in-residence programs place world-class business advice within easy reach of aspiring bioentrepreneurs.


First Rounders Podcast p804

Rachel King

doi:10.1038/nbt0815-804


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Opinion and Comment

Correspondence

Flexible guide-RNA design for CRISPR applications using Protospacer Workbench pp805 - 806

Cameron Ross MacPherson & Artur Scherf

doi:10.1038/nbt.3291


Health ROI as a measure of misalignment of biomedical needs and resources pp807 - 811

Lixia Yao, Ying Li, Soumitra Ghosh, James A Evans & Andrey Rzhetsky

doi:10.1038/nbt.3276


Benchmarking the scientific output of the Innovative Medicines Initiative pp811 - 812

Magda Gunn, Mark Lim, Didi Cross & Michel Goldman

doi:10.1038/nbt.3305


What does the current biotech stock market value? pp813 - 814

Laura McNamee & Fred Ledley

doi:10.1038/nbt.3303


Commentary

The smart and gentle microscope pp815 - 818

Nico Scherf & Jan Huisken

doi:10.1038/nbt.3310

New instruments are needed to realize the potential of quantitative and systematic imaging of living samples. But what would such a microscope look like?


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Features

Patents

The emergence of agbiogenerics pp819 - 823

David J Jefferson, Gregory D Graff, Cecilia L Chi-Ham & Alan B Bennett

doi:10.1038/nbt.3306

Although the first major agbiotech product patent has expired, regulatory requirements could continue to allot a significant degree of control to the original right holder.



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News and Views

Deep learning for regulatory genomics pp825 - 826

Yongjin Park & Manolis Kellis

doi:10.1038/nbt.3313

Computational modeling of DNA and RNA targets of regulatory proteins is improved by a deep-learning approach.

See also: Computational Biology by Alipanahi et al.


A time and a place for sugar in your ears pp827 - 828

Jiahn-Chou Guan & Karen E Koch

doi:10.1038/nbt.3315

Targeting trehalose metabolism improves maize yield under a range of water-deficit conditions.

See also: Research by Nuccio et al.


Research Highlights p828

doi:10.1038/nbt.3307


New PAMs for Cas p829

Markus Elsner

doi:10.1038/nbt.3317


A flexible mesh to record the brain p830

Irene Jarchum

doi:10.1038/nbt.3316


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Computational Biology

Analysis

Predicting the sequence specificities of DNA- and RNA-binding proteins by deep learning pp831 - 838

Babak Alipanahi, Andrew Delong, Matthew T Weirauch & Brendan J Frey

doi:10.1038/nbt.3300

The binding specificities of RNA- and DNA-binding proteins are determined from experimental data using a ‘deep learning’ approach.

See also: News and Views by Park & Kellis


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Research

Brief Communications

Mammalian synthetic circuits with RNA binding proteins for RNA-only delivery pp839 - 841

Liliana Wroblewska, Tasuku Kitada, Kei Endo, Velia Siciliano, Breanna Stillo, Hirohide Saito & Ron Weiss

doi:10.1038/nbt.3301

Using RNA-binding proteins to build genetic circuits enables RNA-only synthetic biology.


Engineered CHO cells for production of diverse, homogeneous glycoproteins pp842 - 844

Zhang Yang, Shengjun Wang, Adnan Halim, Morten Alder Schulz, Morten Frodin, Shamim H Rahman, Malene B Vester-Christensen, Carsten Behrens, Claus Kristensen, Sergey Y Vakhrushev, Eric Paul Bennett, Hans H Wandall & Henrik Clausen

doi:10.1038/nbt.3280

A knockout study of all human glycosyltransferases encoded in CHO cells provides a guide to producing proteins with desired glycosylation patterns.


Articles

Cholangiocytes derived from human induced pluripotent stem cells for disease modeling and drug validation pp845 - 852

Fotios Sampaziotis, Miguel Cardoso de Brito, Pedro Madrigal, Alessandro Bertero, Kourosh Saeb-Parsy, Filipa A C Soares, Elisabeth Schrumpf, Espen Melum, Tom H Karlsen, J Andrew Bradley, William T H Gelson, Susan Davies, Alastair Baker, Arthur Kaser, Graeme J Alexander, Nicholas R F Hannan & Ludovic Vallier

doi:10.1038/nbt.3275

A protocol for generating biliary epithelial cells from human pluripotent stem cells facilitates disease modeling and drug screening.


Directed differentiation of cholangiocytes from human pluripotent stem cells pp853 - 861

Mina Ogawa, Shinichiro Ogawa, Christine E Bear, Saumel Ahmadi, Stephanie Chin, Bin Li, Markus Grompe, Gordon Keller, Binita M Kamath & Anand Ghanekar

doi:10.1038/nbt.3294

A protocol for generating biliary epithelial cells from human pluripotent stem cells facilitates disease modeling and drug screening.


Expression of trehalose-6-phosphate phosphatase in maize ears improves yield in well-watered and drought conditions pp862 - 869

Michael L Nuccio, Jeff Wu, Ron Mowers, Hua-Ping Zhou, Moez Meghji, Lucia F Primavesi, Matthew J Paul, Xi Chen, Yan Gao, Emdadul Haque, Shib Sankar Basu & L Mark Lagrimini

doi:10.1038/nbt.3277

Expression of a single trehalose transgene in maize improves yield in field trials in both well-watered and drought conditions.

See also: News and Views by Guan & Koch


Letters

Visualizing lipid-formulated siRNA release from endosomes and target gene knockdown pp870 - 876

Anders Wittrup, Angela Ai, Xing Liu, Peter Hamar, Radiana Trifonova, Klaus Charisse, Muthiah Manoharan, Tomas Kirchhausen & Judy Lieberman

doi:10.1038/nbt.3298

Live-cell imaging provides new insights in the cytosolic release mechanismsof lipid-formulated siRNAs.


High-throughput sequencing of DNA G-quadruplex structures in the human genome pp877 - 881

Vicki S Chambers, Giovanni Marsico, Jonathan M Boutell, Marco Di Antonio, Geoffrey P Smith & Shankar Balasubramanian

doi:10.1038/nbt.3295

G-quadruplex structures are detected across the human genome using a high-throughput sequencing-based approach.


Corrigenda

Corrigendum: Long-term persistence and development of induced pancreatic beta cells generated by lineage conversion of acinar cells p882

Weida Li, Claudia Cavelti-Weder, Yinying Zhang, Kendell Clement, Scott Donovan, Gabriel Gonzalez, Jiang Zhu, Marianne Stemann, Ke Xu, Tatsu Hashimoto, Takatsugu Yamada, Mio Nakanishi, Yuemei Zhang, Samuel Zeng, David Gifford, Alexander Meissner, Gordon Weir & Qiao Zhou

doi:10.1038/nbt0815-882a


Corrigendum: The ethics of publishing human germline research p882

Arun Sharma & Christopher Thomas Scott

doi:10.1038/nbt0815-882b


Corrigendum: Suppression of vascular permeability and inflammation by targeting of the transcription factor c-Jun p882

Roger G Fahmy, Alla Waldman, Guishui Zhang, Ainslie Mitchell, Nicodemus Tedla, Hong Cai, Carolyn R Geczy, Colin N Chesterman, Michael Perry & Levon M Khachigian

doi:10.1038/nbt0815-882c


Errata

Erratum: A mass-tolerant database search identifies a large proportion of unassigned spectra in shotgun proteomics as modified peptides p882

Joel M Chick, Deepak Kolippakkam, David P Nusinow, Bo Zhai, Ramin Rad, Edward L Huttlin & Steven P Gygi

doi:10.1038/nbt0815-882d


Erratum: Public biotech 2013—the numbers p882

Stacy Lawrence & Riku Lahteenmaki

doi:10.1038/nbt0815-882e


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Careers and Recruitment

Second-quarter biotech job picture p883

Michael Francisco

doi:10.1038/nbt.3314


People

People p884

doi:10.1038/nbt.3320


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