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Using GlycoDelete to produce proteins lacking plant-specific N-glycan modification in seeds

Nature Biotechnology volume 33, pages 1135–1137 (2015)Cite this article

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An Author Correction to this article was published on 05 March 2020

To the Editor:

N-glycosylation is the most widespread posttranslational modification of therapeutic proteins and is essential for proper folding and subunit assembly. However, cells of different species modify N-glycans differently, which can be problematic if a therapeutic protein is expressed in a non-native host cell. For example, glycoproteins produced in yeast are rapidly cleared from the bloodstream and α-galactose moieties produced by many non-human mammalian cell are immunogenic1. Glycosylation requirements often dictate the choice of expression system, with mammalian cells usually preferred2. We use our GlycoDelete technology3 to enable the production of biopharmaceuticals without plant-specific N-glycan modifications in plant seeds.

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Figure 1: N-glycan structures and assembly pathways in wild-type and glycoengineered plants.
Figure 2: Analysis of the WT, cgl and GlycoDelete ASP1.

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Acknowledgements

We thank E. Van Lerberge for the cultivation and propagation of plants, J. Nolf for the purification of the ASP1 proteins and A. Van Hecke for assistance during glycan analysis. The Department of Plant Systems Biology, VIB, Ghent, Belgium. GBP-Fc was kindly provided by T. De Meyer. R.P. and F.S. are supported by predoctoral fellowships from the Agency for Innovation by Science and Technology (IWT) Flanders (Strategic Basic Research fellowship nos. 093450 and 101456). This research was supported by VIB, by Ghent University Industrial Research Fund (UGent-IOF) Advanced Grant no. 041 (N.C.), by FWO research projects Grant no. G.0.541.08.N.10 (N.C.) and G0C9714N (A.D.), and by the European Cooperation in Science and Technology (COST) action FA0804 for conference reimbursements. We also thank the European Cooperation in Science and Technology (COST) action FA0804 for conference reimbursements.

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Author notes

  1. Robin Piron and Francis Santens: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Plant Systems Biology, VIB, Ghent, Belgium

    Robin Piron, Annelies De Paepe & Ann Depicker

  2. Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium

    Robin Piron, Annelies De Paepe & Ann Depicker

  3. Medical Biotechnology Center, VIB, Ghent, Belgium

    Francis Santens & Nico Callewaert

  4. Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium

    Francis Santens & Nico Callewaert

Authors
  1. Robin Piron
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  2. Francis Santens
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  4. Ann Depicker
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  5. Nico Callewaert
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Corresponding authors

Correspondence to Ann Depicker or Nico Callewaert.

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Piron, R., Santens, F., De Paepe, A. et al. Using GlycoDelete to produce proteins lacking plant-specific N-glycan modification in seeds. Nat Biotechnol 33, 1135–1137 (2015). https://doi.org/10.1038/nbt.3359

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