Table of contents


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Editorial

Longitudinal thinking p177

doi:10.1038/nbt.2534

Increasing public and private sector cooperation to support long-term, longitudinal population studies is critical to advance translational research on complex diseases.


Antisense battles small molecule for slice of rare lipid disorder market pp179 - 180

Gunjan Sinha

doi:10.1038/nbt0313-179


Industry pursues co-stimulatory receptor immunomodulators to treat cancer pp181 - 183

Cormac Sheridan

doi:10.1038/nbt0313-181


Amgen fined $762 million p182

Melanie Senior

doi:10.1038/nbt0313-182


Biotech bonanza hits UK p183

Nuala Moran

doi:10.1038/nbt0313-183


FDA okays IT-powered trial p184

Laura DeFrancesco

doi:10.1038/nbt0313-184a


Personalized, sequencing-based immune profiling spurs startups pp184 - 186

Michael Eisenstein

doi:10.1038/nbt0313-184b


Another blow for ALS p185

Gunjan Sinha

doi:10.1038/nbt0313-185


SBIR widens cash net p186

Jeffrey L Fox

doi:10.1038/nbt0313-186a


Drug-proof grapefruit p186

doi:10.1038/nbt0313-186b


Blockbuster dreams for Pfizer's CDK inhibitor p187

Malini Guha

doi:10.1038/nbt0313-187a


Around the world in a month p187

doi:10.1038/nbt0313-187b


Water stress survivors p188

Anna Meldolesi

doi:10.1038/nbt0313-188a


Wyss Institute to rise in Merck Serono shell p188

Nuala Moran

doi:10.1038/nbt0313-188b


Editor's Pick

Orphazyme p189

Ken Garber

doi:10.1038/nbt0313-189

This Danish startup intends to use a chaperone protein to treat lysosomal storage disorders.


Data Page

Drug pipeline: Q412 p190

Laura DeFrancesco

doi:10.1038/nbt.2525


News Feature

Back on target pp191 - 193

Sarah Webb

doi:10.1038/nbt.2520

Recent clinical success of antibody-drug conjugates is resparking interest in the technology for tackling unmet cancer needs. Sarah Webb investigates.


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Bioentrepreneur

Data Page

Innovative startups 2012 p194

Brady Huggett

doi:10.1038/nbt.2518


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Opinion and Comment

Correspondence

Life sciences venture capital in emerging markets pp195 - 201

Justin Chakma, Stephen M Sammut & Ajay Agrawal

doi:10.1038/nbt.2529


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Features

Patents

Patent landscaping for life sciences innovation: toward consistent and transparent practices pp202 - 206

Tania Bubela, E Richard Gold, Gregory D Graff, Daniel R Cahoy, Dianne Nicol & David Castle

doi:10.1038/nbt.2521

As industry, governments and academia increasingly rely on patent landscapes to map scientific and technological trends, an interdisciplinary workshop provides recommendations for developing consistent and transparent landscaping practices.



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News and Views

RNA guides genome engineering pp208 - 209

Claudio Mussolino & Toni Cathomen

doi:10.1038/nbt.2527

The Cas9 endonuclease is reprogrammed by RNAs for site-specific modification of eukaryotic and microbial genomes.

See also: Research by Hwang et al. | Research by Cho et al. | Research by Jiang et al.


Cardiac regenerative medicine 2.0 pp209 - 211

Boon-Seng Soh, Hao Wu & Kenneth R Chien

doi:10.1038/nbt.2524

The key challenge in the field of molecular and cell-based therapies for heart regeneration is controlled delivery.


Single-cell sequencing in its prime pp211 - 212

Roger S Lasken

doi:10.1038/nbt.2523

A new method for amplifying DNA allows more-uniform sequencing coverage of the genome of a single cell.


RESEARCH HIGHLIGHTS p212

doi:10.1038/nbt.2532


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Computational Biology

Analysis

Sensitive detection of somatic point mutations in impure and heterogeneous cancer samples pp213 - 219

Kristian Cibulskis, Michael S Lawrence, Scott L Carter, Andrey Sivachenko, David Jaffe, Carrie Sougnez, Stacey Gabriel, Matthew Meyerson, Eric S Lander & Gad Getz

doi:10.1038/nbt.2514

The MuTect algorithm for calling somatic point mutations enables subclonal analysis of the whole-genome or whole-exome sequencing data being generated in large-scale cancer genomics projects.


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Research

Perspective

Scientific considerations in the review and approval of generic enoxaparin in the United States pp220 - 226

Sau Lee, Andre Raw, Lawrence Yu, Robert Lionberger, Naiqi Ya, Daniela Verthelyi, Amy Rosenberg, Steve Kozlowski, Keith Webber & Janet Woodcock

doi:10.1038/nbt.2528


Brief Communications

Efficient genome editing in zebrafish using a CRISPR-Cas system pp227 - 229

Woong Y Hwang, Yanfang Fu, Deepak Reyon, Morgan L Maeder, Shengdar Q Tsai, Jeffry D Sander, Randall T Peterson, J-R Joanna Yeh & J Keith Joung

doi:10.1038/nbt.2501

A CRISPR-Cas system allows editing of the genome in zebrafish embryos.

See also: News and Views by Mussolino & Cathomen


Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease pp230 - 232

Seung Woo Cho, Sojung Kim, Jong Min Kim & Jin-Soo Kim

doi:10.1038/nbt.2507

The genome of human cells is edited using the bacterial RNA-guided Cas9 endonuclease.

See also: News and Views by Mussolino & Cathomen


Articles

RNA-guided editing of bacterial genomes using CRISPR-Cas systems pp233 - 239

Wenyan Jiang, David Bikard, David Cox, Feng Zhang & Luciano A Marraffini

doi:10.1038/nbt.2508

A CRISPR-Cas system is harnessed to introduce template-driven mutations in S. pneumoniae and E. coli at high efficiency without requiring selectable markers.

See also: News and Views by Mussolino & Cathomen


Draft genome sequence of chickpea (Cicer arietinum) provides a resource for trait improvement Open pp240 - 246

Rajeev K Varshney, Chi Song, Rachit K Saxena, Sarwar Azam, Sheng Yu, Andrew G Sharpe, Steven Cannon, Jongmin Baek, Benjamin D Rosen, Bunyamin Tar'an, Teresa Millan, Xudong Zhang, Larissa D Ramsay, Aiko Iwata, Ying Wang, William Nelson, Andrew D Farmer, Pooran M Gaur, Carol Soderlund, R Varma Penmetsa, Chunyan Xu, Arvind K Bharti, Weiming He, Peter Winter, Shancen Zhao, James K Hane, Noelia Carrasquilla-Garcia, Janet A Condie, Hari D Upadhyaya, Ming-Cheng Luo, Mahendar Thudi, C L L Gowda, Narendra P Singh, Judith Lichtenzveig, Krishna K Gali, Josefa Rubio, N Nadarajan, Jaroslav Dolezel, Kailash C Bansal, Xun Xu, David Edwards, Gengyun Zhang, Guenter Kahl, Juan Gil, Karam B Singh, Swapan K Datta, Scott A Jackson, Jun Wang & Douglas R Cook

doi:10.1038/nbt.2491

A draft sequence of the staple crop kabuli chickpea, together with resequencing and analysis of 90 additional lines from 10 countries, provides a resource for breeders.


Letter

Unfoldase-mediated protein translocation through an α-hemolysin nanopore pp247 - 250

Jeff Nivala, Douglas B Marks & Mark Akeson

doi:10.1038/nbt.2503

Proteins are denatured and threaded through a nanopore by a bacterial unfoldase, allowing certain features of the protein sequence to be detected.


Resources

A library of TAL effector nucleases spanning the human genome pp251 - 258

Yongsub Kim, Jiyeon Kweon, Annie Kim, Jae Kyung Chon, Ji Yeon Yoo, Hye Joo Kim, Sojung Kim, Choongil Lee, Euihwan Jeong, Eugene Chung, Doyoung Kim, Mi Seon Lee, Eun Mi Go, Hye Jung Song, Hwangbeom Kim, Namjin Cho, Duhee Bang, Seokjoong Kim & Jin-Soo Kim

doi:10.1038/nbt.2517

A collection of TALENs targeted to 18,740 human protein-coding genes will facilitate genetic engineering of human cells.


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Careers and Recruitment

Pay increases for nonfounder executives at private life sciences companies rebound pp259 - 261

Bruce Rychlik

doi:10.1038/nbt.2526

Executive cash compensation data for nonfounders revealed a 3.5% year-over-year rise.


People

People p262

doi:10.1038/nbt.2535


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