Full-length mRNA-Seq from single-cell levels of RNA and individual circulating tumor cells

Journal name:
Nature Biotechnology
Volume:
30,
Pages:
777–782
Year published:
DOI:
doi:10.1038/nbt.2282
Received
Accepted
Published online

Abstract

Genome-wide transcriptome analyses are routinely used to monitor tissue-, disease- and cell type–specific gene expression, but it has been technically challenging to generate expression profiles from single cells. Here we describe a robust mRNA-Seq protocol (Smart-Seq) that is applicable down to single cell levels. Compared with existing methods, Smart-Seq has improved read coverage across transcripts, which enhances detailed analyses of alternative transcript isoforms and identification of single-nucleotide polymorphisms. We determined the sensitivity and quantitative accuracy of Smart-Seq for single-cell transcriptomics by evaluating it on total RNA dilution series. We found that although gene expression estimates from single cells have increased noise, hundreds of differentially expressed genes could be identified using few cells per cell type. Applying Smart-Seq to circulating tumor cells from melanomas, we identified distinct gene expression patterns, including candidate biomarkers for melanoma circulating tumor cells. Our protocol will be useful for addressing fundamental biological problems requiring genome-wide transcriptome profiling in rare cells.

At a glance

Figures

  1. Smart-Seq read coverage across transcripts.
    Figure 1: Smart-Seq read coverage across transcripts.

    (a) Comparison of read coverage over transcripts for Smart-Seq–analyzed mouse oocytes (n = 3) and previously published mouse oocyte transcriptome data (ref. 7; n = 2). Transcripts were grouped according to annotated lengths and analyzed separately, with the transcript length ranges indicated (top right). We display the read coverage over the transcripts as a distance from the 3′ end, with the vertical dashed gray line showing the length of the shortest included transcripts after which a decline in read coverage is expected. Error bars represent s.d. among biological replicates. (b) Mean read coverage over transcripts for Smart-Seq data generated from diluted amounts of mouse brain RNA. Independent dilution series (including data from different laboratories) are shown as separate data sets, and sample numbers are listed from uppermost line down. For comparison, we included data from standard mRNA-Seq on 100 ng of mouse brain RNA (non-amplified). Errors bars, s.d. (n = 5, 3, 4 and 4 for lines top to bottom). (c) Read coverage (as in b) for 12 individual human cells of prostate and bladder cancer line origin, analyzed using Smart-Seq (cancer cells; n = 12) and for prostate cell line LNCaP analyzed with standard mRNA-Seq (non-amplified; n = 4). Error bars, s.d.

  2. Sensitivity and variability in Smart-Seq from few or single cells.
    Figure 2: Sensitivity and variability in Smart-Seq from few or single cells.

    (a) Percentage of genes reproducibly detected in replicate pairs, binned according to expression level. We performed all pair-wise comparisons within groups of replicates and report the mean and 90% confidence interval. We used Smart-Seq data generated from diluted amounts of human UHRR total RNA as indicated. As controls, we added both a comparison of technical replicates of human UHRR analyzed using standard mRNA-Seq protocols with 100 ng input RNA (non-amplified) as well as a comparison of human UHRR and brain RNA from standard mRNA-Seq data. (b) Percentage of genes reproducibly detected within replicate pairs, binned according to expression level (as in a) for human LNCaP, PC3 and T24 cells. We show pair-wise comparisons among single cells from the same cancer cell line (blue), among multiple cells of the same cell line (purple and blue), and comparisons among single cells from different cancer cell lines (yellow). (c) Standard deviation in gene expression estimates within replicates in bins of genes sorted according to expression levels. Error bars, s.e.m. (n ≥ 10) (d) Standard deviation in gene expression estimates in replicates (as in c). (eg) Scatter plots showing the relative differences between human UHRR and brain gene expression levels estimated from standard mRNA-Seq data on 100 ng input RNA (x axis) and Smart-Seq generated data (y axis) starting from 1 ng total RNA (e), 100 pg total RNA (f) and 10 pg total RNA (g). Correlation coefficients computed from log2 transformed relative gene expression profiles, together with nonlinear loess regression curves (green) and y = x lines (red).

  3. Transcriptional and post-transcriptional analyses of cancer cell line cells using Smart-Seq.
    Figure 3: Transcriptional and post-transcriptional analyses of cancer cell line cells using Smart-Seq.

    (a) Categorization of individual cells according to cell line of origin using single-cell Smart-Seq transcriptomes. Singular-value decomposition (SVD) analysis was conducted for 12 individual cancer cells (four cells each from the PC3, LNCaP and T24 cancer cell lines) based on global gene expression profiles. Projections are shown based on the first two dimensions that capture most of the variance. The numbers of significantly differentially expressed genes per pair-wise cell line comparison are shown next to the arrows (P < 0.05, 1-way ANOVA and Tukey post-hoc test). (b) Mean number of exons with sufficient read coverage for MISO analyses of exon inclusion levels in sequence-depth matched single-cell mRNA-Seq data. Smart-Seq data from diluted mouse brain RNA (green) compared with previously published mouse ESCs8 and ESC-derived cells (red) and 12 Smart-Seq–analyzed individual prostate and bladder cell line cells (purple). Individual RNA or cell measurements are plotted. (c) Single-cell Smart-Seq reads mapping to a portion of the NEDD4L gene locus from four individual T24 and LNCaP cells. Read coverage is shown as a heatmap with darker blue indicating higher read coverage. (d) Number of differentially included exons identified among the PC3, LNCaP and T24 cell lines from single-cell Smart-Seq analysis on four cells per cell line as a function of estimated false discovery rate.

  4. Single-cell transcriptomes of circulating tumor cells.
    Figure 4: Single-cell transcriptomes of circulating tumor cells.

    (a) Hierarchical clustering of human samples based on gene expression of highly expressed genes (>100 RPKM). Coloring indicates high-order clusters and the confidence in clusters are indicated with bootstrap values (percentage). Samples analyzed include human immune samples (Burkitt's lymphoma cell lines BL41 and BJAB, and white blood cells and lymph node samples) and cells from putative melanoma CTCs (CTC), primary melanocytes (PM), melanoma cell lines SKMEL5 (SKMEL) and UACC257 (UACC), prostate cancer cell lines (LNCaP and PC3), bladder cancer cell line (T24) and human embryonic stem cells (ESC). (b) Expression of melanocyte makers (PMEL, MITF, TYR and MLANA) and immune marker PTPRC in single-cell transcriptomes from a with Burkitt's lymphoma cell lines BL41 and BJAB (BL). (c) Gene expression levels in CTCs for an unbiased set of 100 immune and melanoma markers. (df) Heatmaps showing relative expression of melanoma associated tumor antigens (d), upregulated plasma-membrane proteins (e), and downregulated plasma-membrane proteins (f) in single-cell transcriptomes as in b with the addition of more immune samples (W, white blood cells; L, lymph node). (g) Number of reads from individual PMs and putative CTCs that support the reference (G) or risk (A) allele for the melanoma-associated SNP (rs1126809).

Accession codes

Primary accessions

Gene Expression Omnibus

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Author information

  1. These authors contributed equally to this work.

    • Daniel Ramsköld &
    • Shujun Luo

Affiliations

  1. Ludwig Institute for Cancer Research, Stockholm, Sweden.

    • Daniel Ramsköld,
    • Qiaolin Deng,
    • Omid R Faridani &
    • Rickard Sandberg
  2. Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.

    • Daniel Ramsköld &
    • Rickard Sandberg
  3. Illumina, Inc., Hayward, California, USA.

    • Shujun Luo,
    • Robin Li,
    • Irina Khrebtukova &
    • Gary P Schroth
  4. Department of Chemical Physiology, Center for Regenerative Medicine, The Scripps Research Institute, San Diego, La Jolla, California, USA.

    • Yu-Chieh Wang &
    • Jeanne F Loring
  5. Rebecca and John Moores Cancer Center, San Diego, La Jolla, California, USA.

    • Gregory A Daniels
  6. Department of Reproductive Medicine, University of California, San Diego, La Jolla, California, USA.

    • Louise C Laurent

Contributions

D.R. designed and performed the computational analyses of sequencing reads, prepared figures, tables and methods, and contributed manuscript text. S.L. and R.L. developed protocols and created libraries. I.K. and S.L. did primary data analysis. Y.-C.W., G.A.D. and J.F.L. prepared melanoma circulating tumor cells, melanocytes and melanoma cell line cells. O.R.F. and Q.D. contributed additional sequencing libraries. L.C.L. and G.P.S. contributed to study design and manuscript text. R.S. designed the study and prepared the manuscript, with input from other authors.

Competing financial interests

S.L., R.L., I.K. and G.P.S. are employees and shareholders of Illumina.

Corresponding author

Correspondence to:

Author details

Supplementary information

PDF files

  1. Supplementary Text and Figures (1M)

    Supplementary Figs. 1–11

Excel files

  1. Supplementary Table 1 (45K)

    List of Smart-Seq and standard mRNA-Seq data generated

  2. Supplementary Table 2 (16K)

    List of studies reporting total RNA amount per cell for different mammalian cell types

  3. Supplementary Table 3 (45K)

    List of exons with significantly different inclusion levels in cancer cell line cells

  4. Supplementary Table 4 (5M)

    Differentially expressed genes between circulating tumor cells, primary melanocytes and melanoma cell lines

  5. Supplementary Table 5 (16K)

    Functional categories enriched among differentially expressed genes

Additional data