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References
Zamecnik, P.C. & Stephenson, M.L. Proc. Natl. Acad. Sci. USA 75, 280–284 (1978).
Fire, A. et al. Nature 391, 806–811 (1998).
Henry, S. et al. Toxicologic properties of 2′-methoxyethyl chimeric antisense inhibitors in animals and man. in Antisense Drug Technology: Principles Strategies, and Applications. (ed. Crooke, S.T.) 327–364 (CRC Press, 2007).
Sheehan, J.P. & Phan, T.M. Biochemistry 40, 4980–4989 (2001).
Bennett, C.F. & Swayze, E.E. Annu. Rev. Pharmacol. Toxicol. 50, 259–293 (2010).
Burchard, J. et al. RNA 15, 308–315 (2009).
Jackson, A.L. et al. RNA 12, 1179–1187 (2006).
Snøve, O. & Holen, T. Biochem. Biophys. Res. Commun. 319, 256–263 (2004).
Jackson, A.L. et al. Nat. Biotechnol. 21, 635–637 (2003).
Schwarz, D.S. et al. PLoS Genet. 2, e140 (2006).
Obad, S. et al. Nat. Genet. 43, 371–378 (2011).
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The authors are employees in, or consultants for, commercial companies actively developing oligonucleotides as drugs. See affiliation section for details.
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Lindow, M., Vornlocher, HP., Riley, D. et al. Assessing unintended hybridization-induced biological effects of oligonucleotides. Nat Biotechnol 30, 920–923 (2012). https://doi.org/10.1038/nbt.2376
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DOI: https://doi.org/10.1038/nbt.2376
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