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Assessing unintended hybridization-induced biological effects of oligonucleotides

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Figure 1: Assessment of hybridization-based off-target effects starts with a mechanism- and chemistry-dependent in silico screen, which is interpreted and validated on a case-by-case basis using auxiliary information and experiments.

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Correspondence to Arthur A Levin.

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The authors are employees in, or consultants for, commercial companies actively developing oligonucleotides as drugs. See affiliation section for details.

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Lindow, M., Vornlocher, HP., Riley, D. et al. Assessing unintended hybridization-induced biological effects of oligonucleotides. Nat Biotechnol 30, 920–923 (2012). https://doi.org/10.1038/nbt.2376

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