We report the construction and analysis of 4,836 heterozygous diploid deletion mutants covering 98.4% of the fission yeast genome providing a tool for studying eukaryotic biology. Comprehensive gene dispensability comparisons with budding yeast—the only other eukaryote for which a comprehensive knockout library exists—revealed that 83% of single-copy orthologs in the two yeasts had conserved dispensability. Gene dispensability differed for certain pathways between the two yeasts, including mitochondrial translation and cell cycle checkpoint control. We show that fission yeast has more essential genes than budding yeast and that essential genes are more likely than nonessential genes to be present in a single copy, to be broadly conserved and to contain introns. Growth fitness analyses determined sets of haploinsufficient and haploproficient genes for fission yeast, and comparisons with budding yeast identified specific ribosomal proteins and RNA polymerase subunits, which may act more generally to regulate eukaryotic cell growth.
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- Supplementary Text and Figures (5M)
Supplementary Tables 2–10,12–14,18 Supplementary Figs. 1–10 and Supplementary Methods
- Supplementary Data 2 (19M)
Mapping of the deletions
- Supplementary Data 3 (232K)
Sequence of KanMX4
- Supplementary Table 1 (2M)
The 4,836 deletion set in fission yeast and its genome dataset as a reference (4,914)
- Supplementary Table 11 (576K)
Spreadsheet of 2,438 'one to one' orthologous pairs in fission yeast and budding yeast (for details, see attached Excel file)
- Supplementary Table 15 (1M)
Growth fitness data of S. pombe heterozygous deletion mutants in rich YE media
- Supplementary Table 16 (264K)
List of slow growers from the two yeasts, whose relative fitness is less than 0.97
- Supplementary Table 17 (220K)
List of haploinsufficient (lowest 3% ranked by RF) and haploproficient (highest 3% ranked by RF) genes from the two yeasts in rich media
- Supplementary Data 1 (3M)
All the primer set for the construction of deletion strains
- Supplementary Data 4 (2M)
Design of Affymertix custom GeneChip
- Supplementary Data 5 (10M)
Microarray data set for growth profiling