Trends in biotech literature 2008 - p789

Andrew Marshall

doi:10.1038/nbt0909-789

Full Text - Trends in biotech literature 2008 | PDF (213 KB) - Trends in biotech literature 2008

Trading on hope - pp790 - 792

Jane Qiu

doi:10.1038/nbt0909-790

Jane Qiu investigates the thriving business of selling stem cell transplants as cure-alls for debilitating diseases.

Abstract - Trading on hope | Full Text - Trading on hope | PDF (228 KB) - Trading on hope | Supplementary information

Playing to win - pp793 - 796

Raymond Sekula, Sasha Bakhru & Stefan Zappe

doi:10.1038/bioe.2009.7

PDF (166 KB) - Playing to win

Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray - pp797 - 799

Robert A Childs, Angelina S Palma, Steve Wharton, Tatyana Matrosovich, Yan Liu, Wengang Chai, Maria A Campanero-Rhodes, Yibing Zhang, Markus Eickmann, Makoto Kiso, Alan Hay, Mikhail Matrosovich & Ten Feizi

doi:10.1038/nbt0909-797

Full Text - Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray | PDF (311 KB) - Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray | Supplementary information

Preventing the misuse of gene synthesis - pp800 - 801

Jeremy Minshull & Ralf Wagner

doi:10.1038/nbt0909-800

Full Text - Preventing the misuse of gene synthesis | PDF (205 KB) - Preventing the misuse of gene synthesis

Reply to Preventing the misuse of gene synthesis - p801

doi:10.1038/nbt0909-801a

Full Text - Reply to Preventing the misuse of gene synthesis | PDF (159 KB) - Reply to Preventing the misuse of gene synthesis

Commercialized GM crops and yield - pp801 - 802

Doug Gurian-Sherman

doi:10.1038/nbt0909-801b

Full Text - Commercialized GM crops and yield | PDF (170 KB) - Commercialized GM crops and yield

Commercialized GM crops and yield - pp802 - 803

David Schubert

doi:10.1038/nbt0909-802

Full Text - Commercialized GM crops and yield | PDF (168 KB) - Commercialized GM crops and yield

Reply to Commercialized GM crops and yield - p803

doi:10.1038/nbt0909-803a

Full Text - Reply to Commercialized GM crops and yield | PDF (157 KB) - Reply to Commercialized GM crops and yield

Commercialized GM crops and yield - pp803 - 804

Matin Qaim, Arjunan Subramanian & Prakash Sadashivappa

doi:10.1038/nbt0909-803b

Full Text - Commercialized GM crops and yield | PDF (209 KB) - Commercialized GM crops and yield

The neonatal blood-brain barrier is functionally effective, and immaturity does not explain differential targeting of AAV9 - pp804 - 805

Norman R Saunders, C Joakim Ek & Katarzyna M Dziegielewska

doi:10.1038/nbt0909-804

Full Text - The neonatal blood-brain barrier is functionally effective, and immaturity does not explain differential targeting of AAV9 | PDF (147 KB) - The neonatal blood-brain barrier is functionally effective, and immaturity does not explain differential targeting of AAV9

Reply to The neonatal blood-brain barrier is functionally effective, and immaturity does not explain differential targeting of AAV9 - p805

doi:10.1038/nbt0909-805

Full Text - Reply to The neonatal blood-brain barrier is functionally effective, and immaturity does not explain differential targeting of AAV9 | PDF (290 KB) - Reply to The neonatal blood-brain barrier is functionally effective, and immaturity does not explain differential targeting of AAV9

Accelerated patent examination procedures spur Japanese university innovation - pp815 - 818

John A Tessensohn & Shusaku Yamamoto

doi:10.1038/nbt0909-815

Two recent events—the issuance of the world's first patent for induced pluripotent stem cells and, under a pilot system, the issuance of the fastest patent ever granted—signal a watershed in Japanese academia's transition from gown to town.

Abstract - Accelerated patent examination procedures spur Japanese university innovation | Full Text - Accelerated patent examination procedures spur Japanese university innovation | PDF (337 KB) - Accelerated patent examination procedures spur Japanese university innovation

Recent patent applications in drug screening - p819

doi:10.1038/nbt0909-819

Full Text - Recent patent applications in drug screening | PDF (49 KB) - Recent patent applications in drug screening

Genome-wide identification of post-translational modulators of transcription factor activity in human B cells - pp829 - 837

Kai Wang, Masumichi Saito, Brygida C Bisikirska, Mariano J Alvarez, Wei Keat Lim, Presha Rajbhandari, Qiong Shen, Ilya Nemenman, Katia Basso, Adam A Margolin, Ulf Klein, Riccardo Dalla-Favera & Andrea Califano

doi:10.1038/nbt.1563

Modulatory proteins ensure that transcription factors are active when and where they should be. Wang et al. describe an algorithm for identifying modulators from a compendium of gene expression profiles and experimentally validate four diverse modulators of the MYC oncogene in human B cells.

Abstract - Genome-wide identification of post-translational modulators of transcription factor activity in human B cells | Full Text - Genome-wide identification of post-translational modulators of transcription factor activity in human B cells | PDF (2,400 KB) - Genome-wide identification of post-translational modulators of transcription factor activity in human B cells | Supplementary information

Systemic administration of optimized aptamer-siRNA chimeras promotes regression of PSMA-expressing tumors - pp839 - 846

Justin P Dassie, Xiu-ying Liu, Gregory S Thomas, Ryan M Whitaker, Kristina W Thiel, Katie R Stockdale, David K Meyerholz, Anton P McCaffrey, James O McNamara II & Paloma H Giangrande

doi:10.1038/nbt.1560

Systemic delivery remains a major obstacle in therapeutic applications of siRNAs. Using RNA aptamer–siRNA chimeras with enhanced silencing activity and specificity, Dassie et al. achieve regression of xenograft prostate tumors by intraperitoneal injection.

Abstract - Systemic administration of optimized aptamer-siRNA chimeras promotes regression of PSMA-expressing tumors | Full Text - Systemic administration of optimized aptamer-siRNA chimeras promotes regression of PSMA-expressing tumors | PDF (1,071 KB) - Systemic administration of optimized aptamer-siRNA chimeras promotes regression of PSMA-expressing tumors | Supplementary information

See also: News and Views by Toudjarska & de Fougerolles

Single-molecule sequencing of an individual human genome - pp847 - 850

Dmitry Pushkarev, Norma F Neff & Stephen R Quake

doi:10.1038/nbt.1561

Pushkarev et al. present the first human genome sequence obtained using single-molecule sequencing technology. These results demonstrate that human genome sequencing—previously the turf of large sequencing centers—is now within reach of an individual lab in a matter of weeks.

First Paragraph - Single-molecule sequencing of an individual human genome | Full Text - Single-molecule sequencing of an individual human genome | PDF (804 KB) - Single-molecule sequencing of an individual human genome | Supplementary information

See also: News and Views by Li & Wang

Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases - pp851 - 857

Dirk Hockemeyer, Frank Soldner, Caroline Beard, Qing Gao, Maisam Mitalipova, Russell C DeKelver, George E Katibah, Ranier Amora, Elizabeth A Boydston, Bryan Zeitler, Xiangdong Meng, Jeffrey C Miller, Lei Zhang, Edward J Rebar, Philip D Gregory, Fyodor D Urnov & Rudolf Jaenisch

doi:10.1038/nbt.1562

Hockemeyer et al. demonstrate targeted genetic modification of three genes in human embryonic stem cells and induced pluripotent stem cells using zinc-finger nucleases delivered on plasmids. They use the approach to generate a reporter cell line that monitors the pluripotent state, a drug-inducible overexpression system, and a reporter cell line for a gene that is not expressed in pluripotent stem cells.

Abstract - Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases | Full Text - Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases | PDF (1,189 KB) - Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases | Supplementary information

See also: News and Views by Elefanty & Stanley

Sensitive digital quantification of DNA methylation in clinical samples - pp858 - 863

Meng Li, Wei-dong Chen, Nickolas Papadopoulos, Steven N Goodman, Niels Christian Bjerregaard, Søren Laurberg, Bernard Levin, Hartmut Juhl, Nadir Arber, Helen Moinova, Kris Durkee, Kerstin Schmidt, Yiping He, Frank Diehl, Victor E Velculescu, Shibin Zhou, Luis A Diaz Jr, Kenneth W Kinzler, Sanford D Markowitz & Bert Vogelstein

doi:10.1038/nbt.1559

Gene-specific changes in DNA methylation are promising biomarkers, but sensitive quantitative detection of these epigenetic marks remains challenging. Li et al. adapt the so-called BEAMing technology to enable high-throughput digital quantification of gene methylation in clinical samples.

First Paragraph - Sensitive digital quantification of DNA methylation in clinical samples | Full Text - Sensitive digital quantification of DNA methylation in clinical samples | PDF (596 KB) - Sensitive digital quantification of DNA methylation in clinical samples | Supplementary information

Corrigendum: Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins - p864

Bernd Wollscheid, Damaris Bausch-Fluck, Christine Henderson, Robert O'Brien, Miriam Bibel, Ralph Schiess, Ruedi Aebersold & Julian D Watts

doi:10.1038/nbt0909-864a

Full Text - Corrigendum: Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins | PDF (64 KB) - Corrigendum: Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins

Corrigendum: Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasma - p864

Terri A Addona, Susan E Abbatiello, Birgit Schilling, Steven J Skates, D R Mani, David M Bunk, Clifford H Spiegelman, Lisa J Zimmerman, Amy-Joan L Ham, Hasmik Keshishian, Steven C Hall, Simon Allen, Ronald K Blackman, Christoph H Borchers, Charles Buck, Helene L Cardasis, Michael P Cusack, Nathan G Dodder, Bradford W Gibson, Jason M Held, Tara Hiltke, Angela Jackson, Eric B Johansen, Christopher R Kinsinger, Jing Li, Mehdi Mesri, Thomas A Neubert, Richard K Niles, Trenton C Pulsipher, David Ransohoff, Henry Rodriguez, Paul A Rudnick, Derek Smith, David L Tabb, Tony J Tegeler, Asokan M Variyath, Lorenzo J Vega-Montoto, Åsa Wahlander, Sofia Waldemarson, Mu Wang, Jeffrey R Whiteaker, Lei Zhao, N Leigh Anderson, Susan J Fisher, Daniel C Liebler, Amanda G Paulovich, Fred E Regnier, Paul Tempst & Steven A Carr

doi:10.1038/nbt0909-864b

Full Text - Corrigendum: Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasma | PDF (66 KB) - Corrigendum: Multi-site assessment of the precision and reproducibility of multiple reaction monitoring–based measurements of proteins in plasma

Erratum: Synergistic drug combinations tend to improve therapeutically relevant selectivity - p864

Joseph Lehár, Andrew S Krueger, William Avery, Adrian M Heilbut, Lisa M Johansen, E Roydon Price, Richard J Rickles, Glenn F Short III, Jane E Staunton, Xiaowei Jin, Margaret S Lee, Grant R Zimmermann & Alexis A Borisy

doi:10.1038/nbt0909-864c

Full Text - Erratum: Synergistic drug combinations tend to improve therapeutically relevant selectivity | PDF (64 KB) - Erratum: Synergistic drug combinations tend to improve therapeutically relevant selectivity

Erratum: The Systems Biology Graphical Notation - p864

Nicolas Le Novère, Michael Hucka, Huaiyu Mi, Stuart Moodie, Falk Schreiber, Anatoly Sorokin, Emek Demir, Katja Wegner, Mirit I Aladjem, Sarala M Wimalaratne, Frank T Bergman, Ralph Gauges, Peter Ghazal, Hideya Kawaji, Lu Li, Yukiko Matsuoka, Alice Villéger, Sarah E Boyd, Laurence Calzone, Melanie Courtot, Ugur Dogrusoz, Tom C Freeman, Akira Funahashi, Samik Ghosh, Akiya Jouraku, Sohyoung Kim, Fedor Kolpakov, Augustin Luna, Sven Sahle, Esther Schmidt, Steven Watterson, Guanming Wu, Igor Goryanin, Douglas B Kell, Chris Sander, Herbert Sauro, Jacky L Snoep, Kurt Kohn & Hiroaki Kitano

doi:10.1038/nbt0909-864d

Full Text - Erratum: The Systems Biology Graphical Notation | PDF (65 KB) - Erratum: The Systems Biology Graphical Notation

Erratum: Table of Contents - p864

doi:10.1038/nbt0909-864e

Full Text - Erratum: Table of Contents | PDF (63 KB) - Erratum: Table of Contents

People - p868

doi:10.1038/nbt0909-868

Full Text - People | PDF (88 KB) - People