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Nature Biotechnology 27, 667–670 (1 July 2009) | doi:10.1038/nbt.1550

Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins

Debashish Ray , Hilal Kazan , Esther T Chan , Lourdes Pe|[ntilde]|a Castillo , Sidharth Chaudhry , Shaheynoor Talukder , Benjamin J Blencowe , Quaid Morris & Timothy R Hughes

Metazoan genomes encode hundreds of RNA-binding proteins (RBPs) but RNA-binding preferences for relatively few RBPs have been well defined. Current techniques for determining RNA targets, including in vitro selection and RNA co-immunoprecipitation, require significant time and labor investment.