Brief Communications abstract


Nature Biotechnology 27, 459 - 461 (2009)
Published online: 12 April 2009 | doi:10.1038/nbt.1535

Embryonic stem cell–specific microRNAs promote induced pluripotency

Robert L Judson1, Joshua E Babiarz1, Monica Venere1 & Robert Blelloch1

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This report demonstrates that introduction of microRNAs (miRNAs) specific to embryonic stem cells enhances the production of mouse induced pluripotent stem (iPS) cells. The miRNAs miR-291-3p, miR-294 and miR-295 increase the efficiency of reprogramming by Oct4, Sox2 and Klf4, but not by these factors plus cMyc. cMyc binds the promoter of the miRNAs, suggesting that they are downstream effectors of cMyc during reprogramming. However, unlike cMyc, the miRNAs induce a homogeneous population of iPS cell colonies.

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  1. The Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research, Center for Reproductive Sciences, and Department of Urology, University of California, San Francisco, San Francisco, California, USA.

Correspondence to: Robert Blelloch1 e-mail: blellochr@stemcell.ucsf.edu



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