Deborah Dunsire - p1211

Crispin Littlehales

doi:10.1038/nbt1108-1211

In under three years, Deborah Dunsire transformed Millennium Pharmaceuticals from an unprofitable company to Takeda's oncology centerpiece. What's next?

Abstract - | Full Text - Deborah Dunsire | PDF (102 KB) - Deborah Dunsire

Biotech resilient in Q3 - p1212

Stacy Lawrence

doi:10.1038/nbt1108-1212

Full Text - Biotech resilient in Q3 | PDF (303 KB) - Biotech resilient in Q3

Breaching the barrier - pp1213 - 1215

Alan Dove

doi:10.1038/nbt1108-1213

The blood-brain barrier has confounded the development of many neurological treatments over the years. Now, several companies are claiming they can tackle the problem. Alan Dove reports.

Abstract - | Full Text - Breaching the barrier | PDF (186 KB) - Breaching the barrier

Five IP tips to spread your business wings - pp1217 - 1219

James G Cullem

doi:10.1038/bioe.2008.9

PDF (101 KB) - Five IP tips to spread your business wings

Most gene test sales are misleading - p1221

Helen Wallace

doi:10.1038/nbt1108-1221

Full Text - Most gene test sales are misleading | PDF (139 KB) - Most gene test sales are misleading

Pharming in crop commodities - pp1222 - 1223

C Neal Stewart, Jr

doi:10.1038/nbt1108-1222

Full Text - Pharming in crop commodities | PDF (191 KB) - Pharming in crop commodities

Coexistence in the EU—return of the moratorium on GM crops? - pp1223 - 1225

Yann Devos, Matty Demont & Olivier Sanvido

doi:10.1038/nbt1108-1223

Full Text - Coexistence in the EU—return of the moratorium on GM crops? | PDF (190 KB) - Coexistence in the EU—return of the moratorium on GM crops?

Enforcing pharmaceutical and biotech patent rights in China - pp1235 - 1240

Y Philip Zhang & Michelle M Deng

doi:10.1038/nbt1108-1235

Companies with a significant intellectual property stake in China should put in place an effective protection and enforcement strategy against local and overseas competitors.

Abstract - | Full Text - Enforcing pharmaceutical and biotech patent rights in China | PDF (154 KB) - Enforcing pharmaceutical and biotech patent rights in China

Recent patent applications in sequencing - p1241

doi:10.1038/nbt1108-1241

Full Text - Recent patent applications in sequencing | PDF (49 KB) - Recent patent applications in sequencing

Activity motifs reveal principles of timing in transcriptional control of the yeast metabolic network - pp1251 - 1259

Gal Chechik, Eugene Oh, Oliver Rando, Jonathan Weissman, Aviv Regev & Daphne Koller

doi:10.1038/nbt.1499

Chechik et al. define activity motifs, which extend the concept of a network motif from the static to the dynamic realm. Mapping functional data onto network structure enables them to reveal new systems-level principles describing how yeast cells integrate exogenous signals and use transcriptional regulation to optimize metabolic responses to environmental perturbations.

Abstract - | Full Text - Activity motifs reveal principles of timing in transcriptional control of the yeast metabolic network | PDF (589 KB) - Activity motifs reveal principles of timing in transcriptional control of the yeast metabolic network | Supplementary information

Transdermal drug delivery - pp1261 - 1268

Mark R Prausnitz & Robert Langer

doi:10.1038/nbt.1504

Abstract - | Full Text - Transdermal drug delivery | PDF (330 KB) - Transdermal drug delivery

Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 - pp1269 - 1275

Danwei Huangfu, Kenji Osafune, René Maehr, Wenjun Guo, Astrid Eijkelenboom, Shuibing Chen, Whitney Muhlestein & Douglas A Melton

doi:10.1038/nbt.1502

One strategy for advancing induced pluripotent stem (iPS) cell technology toward the clinic is to replace the reprogramming genes with small molecules. Huangfu et al. show that the HDAC inhibitor valproic acid can substitute for the reprogramming gene Klf4, allowing human iPS cells to be generated with only two transgenes, Oct4 and Sox2.

Abstract - | Full Text - Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 | PDF (1,245 KB) - Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2 | Supplementary information

See also: News and Views by Lowry & Plath

Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes - pp1276 - 1284

Trond Aasen, Angel Raya, Maria J Barrero, Elena Garreta, Antonella Consiglio, Federico Gonzalez, Rita Vassena, Josipa Bili, Vladimir Pekarik, Gustavo Tiscornia, Michael Edel, Stéphanie Boué & Juan Carlos Izpisúa Belmonte

doi:10.1038/nbt.1503

Aasen et al. boost the efficiency of human induced pluripotent stem (iPS) cell generation 100-fold by starting with keratinocytes rather than fibroblasts. They also produce iPS cells from plucked adult hair, an easily accessible source of cells that avoids the need for a biopsy.

Abstract - | Full Text - Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes | PDF (1,833 KB) - Efficient and rapid generation of induced pluripotent stem cells from human keratinocytes | Supplementary information

See also: News and Views by Gadue & Cotsarelis

Protein microarrays with carbon nanotubes as multicolor Raman labels - pp1285 - 1292

Zhuo Chen, Scott M Tabakman, Andrew P Goodwin, Michael G Kattah, Dan Daranciang, Xinran Wang, Guangyu Zhang, Xiaolin Li, Zhuang Liu, Paul J Utz, Kaili Jiang, Shoushan Fan & Hongjie Dai

doi:10.1038/nbt.1501

The picomolar sensitivity of fluorescence-based protein detection limits the use of protein arrays in research and clinical diagnosis. Chen et al. use antibody-tagged single-walled carbon nanotubes as multicolor Raman labels to detect femtomolar levels of serum analytes over a wide dynamic range.

Abstract - | Full Text - Protein microarrays with carbon nanotubes as multicolor Raman labels | PDF (564 KB) - Protein microarrays with carbon nanotubes as multicolor Raman labels | Supplementary information

See also: News and Views by Srivastava & LaBaer

An integrated software system for analyzing ChIP-chip and ChIP-seq data - pp1293 - 1300

Hongkai Ji, Hui Jiang, Wenxiu Ma, David S Johnson, Richard M Myers & Wing H Wong

doi:10.1038/nbt.1505

Analyzing the massive and heterogenous datasets from genome-wide chromatin immunoprecipitation (ChIP) datasets presents several computational and statistical challenges. Ji et al. present a software suite that integrates all steps in ChIP-chip and ChIP-seq data analyses and illustrate the use of these tools by comparing the ability of the two platforms to identify transcription factor binding sites.

Abstract - | Full Text - An integrated software system for analyzing ChIP-chip and ChIP-seq data | PDF (741 KB) - An integrated software system for analyzing ChIP-chip and ChIP-seq data | Supplementary information

Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors - pp1301 - 1308

Eugenio Butelli, Lucilla Titta, Marco Giorgio, Hans-Peter Mock, Andrea Matros, Silke Peterek, Elio G W M Schijlen, Robert D Hall, Arnaud G Bovy, Jie Luo & Cathie Martin

doi:10.1038/nbt.1506

Fruit-specific overexpression of a pair of snapdragon transcription factors produces tomatoes that uniformly accumulate anthocyanins at levels unprecedented for metabolic engineering. When included as a dietary supplement, the purple tomatoes increase the life spans of tumorigenic p53 knockout mice.

First Paragraph - | Full Text - Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors | PDF (524 KB) - Enrichment of tomato fruit with health-promoting anthocyanins by expression of select transcription factors | Supplementary information

A case for consulting - pp1309 - 1310

Mari Paul

doi:10.1038/nbt1108-1309

Today's demographics call for biotech companies to be more flexible with their workforce.

Abstract - | Full Text - A case for consulting | PDF (111 KB) - A case for consulting

People - p1312

doi:10.1038/nbt1108-1312

Full Text - People | PDF (118 KB) - People