Research abstract

Resources abstract


Nature Biotechnology 25, 803 - 816 (2007)
Published online: 17 June 2007 | doi:10.1038/nbt1318

Characterization of human embryonic stem cell lines by the International Stem Cell Initiative

The International Stem Cell Initiative*


The International Stem Cell Initiative characterized 59 human embryonic stem cell lines from 17 laboratories worldwide. Despite diverse genotypes and different techniques used for derivation and maintenance, all lines exhibited similar expression patterns for several markers of human embryonic stem cells. They expressed the glycolipid antigens SSEA3 and SSEA4, the keratan sulfate antigens TRA-1-60, TRA-1-81, GCTM2 and GCT343, and the protein antigens CD9, Thy1 (also known as CD90), tissue-nonspecific alkaline phosphatase and class 1 HLA, as well as the strongly developmentally regulated genes NANOG, POU5F1 (formerly known as OCT4), TDGF1, DNMT3B, GABRB3 and GDF3. Nevertheless, the lines were not identical: differences in expression of several lineage markers were evident, and several imprinted genes showed generally similar allele-specific expression patterns, but some gene-dependent variation was observed. Also, some female lines expressed readily detectable levels of XIST whereas others did not. No significant contamination of the lines with mycoplasma, bacteria or cytopathic viruses was detected.

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  1. UK Stem Cell Bank, Division of Cell Biology and Imaging, National Institute for Biological Standards and Control, South Mimms, Herts., EN6 3QG, UK.
  2. Centre for Stem Cell Biology, Department of Molecular Biology and Biotechnology, The University of Sheffield, Sheffield S10 2TN, UK.
  3. Unit for Molecular Embryology, Department for Laboratory Medicine, Karolinska Institutet, 141 57 Stockholm, Sweden.
  4. Rambam Medical Center, Technion–Israel Institute of Technology, POB 9602, Haifa 31096, Israel.
  5. Centre for Stem Cell Biology, Department of Biomedical Science, The University of Sheffield, Sheffield S10 2TN, UK.
  6. Geneservice Limited, No.2 Cambridge Science Park, Cambridge, CB3 0FE, UK.
  7. Cancer Research UK Genotyping Facility, St James's University Hospital, Leeds, LS9 3LP, UK.
  8. Stem Cell Sciences Ltd., Building 75, Monash University, Wellington Rd., Clayton, Victoria 3800, Australia.
  9. The Hebrew University of Jerusalem, Institute of Life Sciences, Department of Genetics, Edmond J. Safra Campus, Givat Ram, Jerusalem 91904, Israel.
  10. Matrix Technologies, Thermo Fisher Scientific, Unit 2 Lower Meadow Road, Handforth, SK9 3LP, UK.
  11. NIH Stem Cell Unit, Bldg 35/2B-213, MSC 3703, National Institutes of Health, 9000 Rockville Pike, Bethesda, Maryland 20892, USA.
  12. Bioprocessing Technology Institute, 20 Biopolis Way, #06-01 Centros, Singapore 138668.
  13. The Jackson Laboratory, 600 Main Street, Bar Harbor, Maine 04609, USA.
  14. Department of Surgery, University of Cambridge, Addenbrookes Hospital, Hills Road, Cambridge, CB2 2XY, UK.
  15. Department of Pathology, The University of Kansas, School of Medicine, 3901 Rainbow Blvd., Kansas City, Kansas 66160, USA.
  16. Samuel Lunenfeld Research Institute, Room 881, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada, M5G 1X5.
  17. Department of Biology, Faculty of Medicine, Masaryk University, Brno, Czech Republic, and Department of Molecular Embryology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Brno, Czech Republic.
  18. Cellartis AB, Arvid Wallgrens Backe 20, 413 46, Göteborg, Sweden.
  19. Monash Immunology and Stem Cell Laboratories (MISCL), Level 3, STRIP–Building 75, Monash University, Clayton, Victoria, 3800 Australia.
  20. Karolinska Institutet, Dept. of Clinical Science, Intervention and Technology, Div. of Obstetrics/Gynecology, Karolinska University Hospital, Huddinge, SE-141 86 Stockholm, Sweden.
  21. Department of Laboratory Medicine, Clinical Research Centre, Karolinska Institutet, 141 57 Stockholm, Sweden.
  22. Uppsala University, Department of Development and Genetics, SE-7523236, Sweden.
  23. Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology & Regenerative Medicine, Department of Obstetrics and Gynecology, Stanford University, Palo Alto, California USA.
  24. The Jackson Laboratory & the Institute for Molecular Biophysics, 600 Main Street, Bar Harbor, Maine 04609, USA.
  25. Institute of Human Genetics, International Centre for Life, Central Parkway, University of Newcastle, Newcastle upon Tyne, NE1 3BZ, UK.
  26. Hubrecht Laboratory, Uppsalalaan 8, 3584CT Utrecht, The Netherlands.
  27. INSERM UR 525, Faculté de Médecine Pitié-Salpêtrière, Paris, France.
  28. WiCell Research Institute, PO Box 7365, Madison, WI 53707-7365, USA.
  29. WiCell Research Institute, 1298 S Mason Road, St. Louis, Missouri 63131, USA.
  30. Laboratory of Embryonic Stem Cell Biology, Monash Institute of Medical Research, Monash University & the Australian Stem Cell Centre, Wellington Road, Clayton 3800, Victoria, Australia.
  31. Biomedicum Stem Cell Center, Biomedicum Helsinki, PO Box 63 FIN-00014, University of Helsinki, Helsinki, Finland.
  32. Wolfson Centre for Age-Related Diseases, King's College London, London SE1 1UL, UK.
  33. Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
  34. King's College London/University College London, Regenerative Medicine Bioprocessing Unit, The Advanced Centre for Biochemical Engineering, Department of Biochemical Engineering, University College London, Torrington Place, London, WC1E 7JE, UK.
  35. Centre for Reproduction and Development, Monash Institute of Medical Research, Monash University & the Australian Stem Cell Centre, Wellington Road, Clayton 3800, Victoria, Australia.
  36. Center for Stem Cell and Regenerative Medicine, Keck School of Medicine, University of Southern California, c/o Zilkha Neurogenetic Institute, Room 543, 1501 San Pablo Street, Los Angeles, California 90089-282, USA.
  37. Hadassah Human Embryonic Stem Cell Research Center, The Goldyne Savad Institute of Gene Therapy & The Department of Obstetrics and Gynecology, Hadassah University Medical Center, Ein Kerem POB 12000 Jerusalem 91120, Israel.
  38. Novocell, 111 Riverbend Rd., Athens, Georgia 30606, USA.
  39. Developmental Biology Program, The Hospital for Sick Children, TMDT Building, Room 13-305, 101 College Street, Toronto, Ontario, M5G 1L7, Canada.
  40. Program for Developmental Biology. The Hospital for Sick Children, 555 University Avenue, Toronto, Ontario, M5G 1X8, Canada.
  41. Stem Cell Center, Lund University, BMC, B10, Klinikgatan 26, SE-221-84 Lund, Sweden.
  42. Centro de Investigación Príncipe Felipe, Valencia, Spain.

Correspondence to: e-mail: p.w.andrews@sheffield.ac.uk



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