Research abstract
Letter abstract
Nature Biotechnology 25, 473 - 477 (2007)
Published online: 11 March 2007 | Corrected online: 23 March 2007 | doi:10.1038/nbt1291
An expression atlas of rice mRNAs and small RNAs
Kan Nobuta1,2, R C Venu4, Cheng Lu1,2, André Beló2, Kalyan Vemaraju1, Karthik Kulkarni1, Wenzhong Wang1, Manoj Pillay1, Pamela J Green1,2,3, Guo-liang Wang4 & Blake C Meyers1,2
Identification of all expressed transcripts in a sequenced genome is essential both for genome analysis and for realization of the goals of systems biology. We used the transcriptional profiling technology called 'massively parallel signature sequencing' to develop a comprehensive expression atlas of rice (Oryza sativa cv Nipponbare). We sequenced 46,971,553 mRNA transcripts from 22 libraries, and 2,953,855 small RNAs from 3 libraries. The data demonstrate widespread transcription throughout the genome, including sense expression of at least 25,500 annotated genes and antisense expression of nearly 9,000 annotated genes. An additional set of 
15,000 mRNA signatures mapped to unannotated genomic regions. The majority of the small RNA data represented lower abundance short interfering RNAs that match repetitive sequences, intergenic regions and genes. Among these, numerous clusters of highly regulated small RNAs were readily observed. We developed a genome browser (http://mpss.udel.edu/rice) for public access to the transcriptional profiling data for this important crop.
- Delaware Biotechnology Institute, University of Delaware, Newark, Delaware 19711.
- Department of Plant and Soil Sciences, University of Delaware, Newark, Delaware 19711.
- College of Marine and Earth Studies, University of Delaware, Newark, Delaware 19711.
- Department of Plant Pathology, The Ohio State University, Columbus, OH 43210.
Correspondence to: Blake C Meyers1,2 e-mail: meyers@dbi.udel.edu
MORE ARTICLES LIKE THIS
These links to content published by NPG are automatically generated.
NEWS AND VIEWS
Tagging the rice transcriptomeNature Biotechnology News and Views (01 Apr 2007)
Tagging the rice transcriptomeNature Biotechnology News and Views (01 Apr 2007)
RESEARCH
An expression atlas of rice mRNAs and small RNAsNature Biotechnology Research (01 Apr 2007)
Analysis of the transcriptional complexity of Arabidopsis thaliana by massively parallel signature sequencingNature Biotechnology Research (01 Aug 2004)
See all 8 matches for Research
