Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • News & Views
  • Published:

Towards quantitative analysis of proteome dynamics

Nature Biotechnology volume 25, pages 298–300 (2007)Cite this article

Integrating mass-spectrometry data across multiple samples promises to reveal the dynamics of proteomes.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Figure 1: Two key features of the MasterMap approach are (i) the serial dilution of samples to eliminate background noise from immunoprecipitation pull-downs and (ii) the processing of LC-MS spectra from various biological samples by an algorithm that performs multiple alignments of precursor ions from the different LC-MS runs followed by clustering of the MS1 features according to selected intensity profiles.

References

  1. Rinner, O. et al. Nat. Biotechnol. 25, 345–352 (2007).

    Article  CAS  Google Scholar 

  2. Ong, S.E. & Mann, M. Nat. Chem. Biol. 1, 252–262 (2005).

    Article  CAS  Google Scholar 

  3. Ong, S.E., Foster, L.J. & Mann, M. Methods 29, 124–130 (2003).

    Article  CAS  Google Scholar 

  4. Domon, B. & Aebersold, R. Science 312, 212–217 (2006).

    Article  CAS  Google Scholar 

  5. Gygi, S.P. et al. Nat. Biotechnol. 17, 994–999 (1999).

    Article  CAS  Google Scholar 

  6. Li, X.J. et al. Anal. Chem. 76, 3856–3860 (2004).

    Article  CAS  Google Scholar 

  7. Silva, J.C. et al. Mol. Cell. Proteomics 5, 144–156 (2006).

    Article  CAS  Google Scholar 

  8. Wang, W. et al. Anal. Chem. 75, 4818–4826 (2003).

    Article  CAS  Google Scholar 

  9. Andersen, J.S. et al. Nature 426, 570–574 (2003).

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Sebastian Kühner and Anne-Claude Gavin are at the EMBL, Structural and Computational Biology Unit, Meyerhofstrasse 1, D-69117 Heidelberg, Germany. gavin@embl.de,

    Sebastian Kühner & Anne-Claude Gavin

Authors
  1. Sebastian Kühner
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Anne-Claude Gavin
    View author publications

    You can also search for this author in PubMed Google Scholar

Ethics declarations

Competing interests

The authors declare no competing financial interests.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kühner, S., Gavin, AC. Towards quantitative analysis of proteome dynamics. Nat Biotechnol 25, 298–300 (2007). https://doi.org/10.1038/nbt0307-298

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1038/nbt0307-298

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing