Supplementary information
From the following article
ATP and FRET—a cautionary note
Marieke Willemse, Edwin Janssen, Frank de Lange, Bé Wieringa & Jack Fransen
Nature Biotechnology 25, 170 - 172 (2007)
doi:10.1038/nbt0207-170
Supplementary Figure 1
Characteristics of the different sensors initially designed to monitor ATP concentrations.
Supplementary Figure 2
Effect of ATP on FRET ratio is not the result of a change in fluorescent properties of the CFP or YFP fluorophores.
Supplementary Figure 3
The effect of [ATP] on the FRET ratio of the Xa protease cleavage site based control sensor is dependent on the MgCl2 concentration in the buffer.
Supplementary Video 1
Human skin fibroblasts showing the changes in the spectra of the XProt-sensor in time upon washout and addition of ATP.
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