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ATP and FRET—a cautionary note

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Figure 1: ATP concentration affects signal efficacy of CFP-YFP–based FRET sensors.
Figure 2: FRET sensors show ATP concentration–dependent YFP/CFP peak intensity ratio changes in permeabilized cells in vivo.

References

  1. Giepmans, B.N.G. et al. Science 312, 217–224 (2006).

    Article  CAS  Google Scholar 

  2. Nikolaev, V.O., Gambaryan, S. & Lohse, M.J. Nat. Methods 3, 23–25 (2006).

    Article  CAS  Google Scholar 

  3. Janssen, E. et al. EMBO J. 19, 6371–6381 (2000).

    Article  CAS  Google Scholar 

  4. He, L. et al. Nucleic Acids Res. 33, e61 (2005).

    Article  Google Scholar 

  5. Amat, A. et al. J. Photochem. Photobiol. B 81, 26–32 (2005).

    Article  CAS  Google Scholar 

  6. Li, C. et al. Angew. Chem. 117, 6529–6532 (2005).

    Article  Google Scholar 

  7. Butterfield, S.M. & Waters, M.L. J. Am. Chem. Soc. 125, 9580–9581 (2003).

    Article  CAS  Google Scholar 

  8. Selivanov, V.A. et al. Mol. Cell. Biochem. 256/257, 243–256 (2004).

    Article  CAS  Google Scholar 

  9. Vinnakota, K.C. & Bassingthwaighte, J.B. Am. J. Physiol. Heart Circ. Physiol. 286, H1742–H1749 (2004).

    Article  CAS  Google Scholar 

  10. Pivovarenko, V.G., Vadzyuk, O.B. & Kosterin, S.O. J. Fluorescence 16, 9–15 (2006).

    Article  CAS  Google Scholar 

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Acknowledgements

This work was supported by ZonMw HORIZON Breakthrough Grant 050-71-019 and NKB-KWF grant 2002-2763; we thank L. He, St. Jude Children's Research Hospital, for kindly providing us with the CFP-TRAF2TD-YFP construct, and R. Lynch, University of Arizona, for helpful suggestions.

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Supplementary information

Supplementary Figure 1

Characteristics of the different sensors initially designed to monitor ATP concentrations. (PDF 219 kb)

Supplementary Figure 2

Effect of ATP on FRET ratio is not the result of a change in fluorescent properties of the CFP or YFP fluorophores. (PDF 2029 kb)

Supplementary Figure 3

The effect of [ATP] on the FRET ratio of the Xa protease cleavage site based control sensor is dependent on the MgCl2 concentration in the buffer. (PDF 723 kb)

Supplementary Video 1

Human skin fibroblasts showing the changes in the spectra of the XProt-sensor in time upon washout and addition of ATP. (AVI 9089 kb)

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Willemse, M., Janssen, E., Lange, F. et al. ATP and FRET—a cautionary note. Nat Biotechnol 25, 170–172 (2007). https://doi.org/10.1038/nbt0207-170

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