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Reporting neural activity with genetically encoded calcium indicators
Brain Cell Biology Open Access 01 August 2008
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Acknowledgements
This work was supported by ZonMw HORIZON Breakthrough Grant 050-71-019 and NKB-KWF grant 2002-2763; we thank L. He, St. Jude Children's Research Hospital, for kindly providing us with the CFP-TRAF2TD-YFP construct, and R. Lynch, University of Arizona, for helpful suggestions.
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Supplementary information
Supplementary Figure 1
Characteristics of the different sensors initially designed to monitor ATP concentrations. (PDF 219 kb)
Supplementary Figure 2
Effect of ATP on FRET ratio is not the result of a change in fluorescent properties of the CFP or YFP fluorophores. (PDF 2029 kb)
Supplementary Figure 3
The effect of [ATP] on the FRET ratio of the Xa protease cleavage site based control sensor is dependent on the MgCl2 concentration in the buffer. (PDF 723 kb)
Supplementary Video 1
Human skin fibroblasts showing the changes in the spectra of the XProt-sensor in time upon washout and addition of ATP. (AVI 9089 kb)
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Willemse, M., Janssen, E., Lange, F. et al. ATP and FRET—a cautionary note. Nat Biotechnol 25, 170–172 (2007). https://doi.org/10.1038/nbt0207-170
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DOI: https://doi.org/10.1038/nbt0207-170
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